millipore,
密理博,HMMP1MAG-55K,MILLIPLEX MAP Human MMP Magnetic Bead Panel 1 - Immunology Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| MMP-3, MMP-12, MMP-13
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | HMMP1MAG-55K |
| Trade Name |
|
| Description | MILLIPLEX MAP Human MMP Magnetic Bead Panel 1 - Immunology Multiplex Assay |
| Overview | MILLIPLEX MAP Human MMP Magnetic Bead Panel 1 is a 3 plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum/plasma samples: MMP-3, MMP-12 and MMP-13. |
| Background Information | The MMPs (matrix metalloproteinases), a family of zinc proteases responsible for the breakdown of extracellular matrix (ECM), play a key role in normal physiological processes, such as embryonic development and tissue morphogenesis, tissue and bone remodeling, wound healing and angiogenesis. These processes rely on MMPs’ role in the cleavage of cell surface receptors, the release of apoptotic ligands, cell proliferation and differentiation, and chemokine activity modulation. Similar in structure, MMPs are synthesized and secreted as inactive pro-enzymes that require proteolytic cleavage for activation. This process can be mediated by serine proteases or other MMPs. An increase in MMP expression occurs in response to a wide range of stimuli, including adhesion molecules, growth factors, cytokines and hormones. Regulation of MMP activity is controlled primarily by TIMPs (tissue inhibitors of metalloproteinases). Therefore, disruption of the MMP/TIMP balance can result in arthritis, cardiovascular disease and tumor growth and metastasis. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- Intra-assay:< 5
- Inter-assay:< 10
|
| Panel Type | MAGNETIC Cytokines/Chemokines |
| Quality Level | MQ200
|
| Applications
|
| Application | Matrix Metalloproteinase Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous analysis of multiple MMPs biomarkers in human serum, plasma and cell culture samples. |
| Key Applications |
|
| Application Notes | • This panel was optimized when it was converted to the magnetic bead format. The following changes were made to this kit:
o MMP-12 detection antibody
o Standard curve concentrations for MMP-3 and MMP-13
• This is a 2-hour initial incubation assay requiring 25 μL neat human serum and plasma samples. This panel has also been analytically validated in urine, tissue/cell lystate and culture supernatant samples. |
| Biological Information
|
| Specificity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Species Reactivity |
|
| Analytes Available |
|
| Physicochemical Information
|
| Sensitivity |
- See protocol for sensitivities of individual analytes
|
| Standard Curve Range |
- MMP-3: 146 - 150,000 pg/mL
- MMP-12: 98 - 100,000 pg/mL
- MMP-13: 58 - 60,000 pg/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
