millipore,
密理博,HKI3MAG-99K,MILLIPLEX MAP Human Kidney Injury Magnetic Bead Panel 3 - Toxicity Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| β-2-Microglobulin, RBP4, Uromodulin
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | HKI3MAG-99K |
| Trade Name |
|
| Description | MILLIPLEX MAP Human Kidney Injury Magnetic Bead Panel 3 - Toxicity Multiplex Assay |
| Background Information | Maintenance of physiological balance in the body is attributed in part to normal kidney function. Altered kidney function due to injury, drug toxicity or kidney failure may be life threatening. Toxicity is the leading cause of drug failure, so research is expanding in search of more sensitive, rapid methods to determine organ-specific damage as quickly as possible. Traditionally, blood urea nitrogen (BUN) and serum creatinine tests have been used to determine drug-induced damage to the kidney. These tests only detect kidney damage after it begins and do not allow for the elucidation of where in the kidney the damage has occurred. Specificity and earlier detection of kidney injury is vital, and this area of research is expanding in search of more sensitive, rapid methods for determining the specific area of damage in kidney.
EMD millipore provides valuable research assays to investigate multiple biomarkers of kidney injury in human urine samples using the Luminex xMAP® instrument platform. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- Inter-assay: < 15%
- Intra-assay: < 10%
|
| Panel Type | MAGNETIC Toxicity |
| Quality Level | MQ200
|
| Applications
|
| Application | The analytes available for this multiplex kit are: β-2-Microglobulin (β2M), Retinol Binding Protein 4 (RBP4), Uromodulin. |
| Key Applications |
|
| Application Notes | • Customers need to determine the optimal dilution factor for their samples. Our recommendations are based on urine samples from normal subjects.
• These assays require 25 µL diluted urine sample per well, sample dilution recommendations is 1:500 dilution in kit Assay Buffer
• An overnight (4°C) incubation is recommended. |
| Biological Information
|
| Species Reactivity |
|
| Analytes Available |
- β-2-Microglobulin
- RBP4
- Uromodulin
|
| Cross Reactivity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. |
| Accuracy |
|
| Physicochemical Information
|
| Sensitivity |
- 0.052 - 0.175 ng/mL (MinDC = 2SD)
- Please see kit protocol for individual assay sensitivities.
|
| Standard Curve Range |
- β-2-Microglobulin: 0.10 – 100 ng/mL
- RBP4: 0.05 – 50 ng/mL
- Uromodulin: 0.10 – 100 ng/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Packaging Information
|
| Material Size | 96-well plate |
