重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|---|---|---|
| IL-18 | H | Mplex | Luminex xMAP |
| Description | |
|---|---|
| Catalogue Number | HIL18MAG-66K |
| Trade Name |
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| Description | MILLIPLEX MAP Human IL-18 Singleplex Magnetic Bead Kit |
| Overview | EMD millipore’s MILLIPLEX® MAP Human IL-18 Singleplex Kit is validated for detection in serum, plasma or tissue/cell supernatant samples, and for use as follows: • On its own as a single protein quantification assay, Cat. No. HIL18MAG-66K (the standard is calibrated to the WHO standard) • Combined with MILLIPLEX® MAP Human Cytokine/Chemokine Panel 1, Cat. No. HCYTOMAG-60K (or the corresponding premix or bulk format kits) • Combined with MILLIPLEX® MAP Human Cytokine/Chemokine Panel 2, Cat. No. HCYP2MAG-62K (with the exception of the CTACK/CCL27 assay, due to bead region overlap) • Combined with MILLIPLEX® MAP Human Th17 Panel, Cat. No. HTH17MAG-14K (or the corresponding premix or bulk format kits) |
| Background Information | Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a member of the IL-1 cytokine superfamily and acts as a pleiotropic cytokine that modulates both innate and acquired immune responses. Disrupted regulation of IL-18 bioactivity is linked to multiple types of diseases including chronic inflammatory diseases, autoimmune diseases, a variety of cancers, and infectious diseases. It also has been implicated to play a role in emphysema, myocardial function, cardiovascular disease, metabolic syndrome, acute kidney injury and neurodegeneration. Our MILLIPLEX® MAP Human IL-18 Singleplex Magnetic Bead Kit is a single analyte kit to be used for the quantification of IL-18 in serum, plasma, and tissue/cell supernatant samples. The Human IL-18 Singleplex standard is calibrated to the WHO standard. |
| Product Information | |
|---|---|
| Detection method | Luminex xMAP |
| Configuration | Single Plex |
| Precision, % |
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| Panel Type | MAGNETIC Cytokines/Chemokines |
| Applications | |
|---|---|
| Application | This kit is validated for serum, plasma or tissue/cell supernatant samples, and for use as a single protein quantification assay or combined with • Human Cytokine/Chemokine Panel 1 (HCYTOMAG-60K) • Human Cytokine/Chemokine Panel 2 (HCYP2MAG-62K except CTACK/CCL27) • Human Th17 Panel (HTH17MAG-14K) |
| Key Applications |
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| Application Notes | • A maximum of 25 μL per well neat serum or plasma can be used. Tissue culture or other media may be run neat or appropriately diluted in control medium. • As single analyte assay, this assay may be run as a 2 hour or overnight incubation assay. If combining with one of the other indicated panels, please follow the protocol for that particular panel. • When running this assay as a single analyte assay, follow the protocol directions and use the reagents that come in the HIL18MAG-66K kit. • When combining the IL-18 analyte with one of the larger panels, use the reagents provided in the other kit. Carefully reference both protocols to ensure proper procedures are followed, particularly for the following sections: o “PREPARATION OF REAGENTS FOR IMMUNOASSAY: HUMAN IL-18 WITH OTHER KITS” - Note: Use the serum matrix (if needed) provided with the larger panel o Follow the “IMMUNOASSAY PROCEDURE” for the larger panel • If comparing single analyte IL-18 sample values with IL-18 combined with one of the possiible kitss, you must run a curve to determine sample correlation and slope. Differences in sample values are expected due to differences in serum matrix, but should highly correlate. |
| Biological Information | |
|---|---|
| Species Reactivity |
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| Analytes Available |
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| Cross Reactivity | Cross-reactivity is non-detectable or negligible between the antibodies in this kit and the antibodies of the panels it can be combined into: • Human Cytokine/Chemokine Panel 1 (HCYTOMAG-60K or the corresponding premix or bulk format kits) • Human Cytokine/Chemokine Panel 2 (HCYP2MAG-62K (with the exception of the CTACK/CCL27 assay, due to bead region overlap)) • Human Th17 Panel (HTH17MAG-14K or the corresponding premix or bulk format kits) |
| Accuracy |
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| Physicochemical Information | |
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| Sensitivity |
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| Standard Curve Range |
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| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information | |
|---|---|
| Material Size | 96-well plate |
