millipore,
密理博,HIGFBMAG-53K,MILLIPLEX MAP Human IGF Binding Protein Magnetic Bead Panel - Metabolism Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGFBP-7
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | HIGFBMAG-53K |
| Trade Name |
|
| Description | MILLIPLEX MAP Human IGF Binding Protein Magnetic Bead Panel - Metabolism Multiplex Assay |
| Overview | The MILLIPLEX MAP Human IGF Binding Protein (IGFBP) Magnetic Bead Panel, is used for the simultaneous quantification of any or all of the following analytes in human serum, plasma, and cell culture conditioned-media samples: IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, and IGFBP-7 |
| Background Information | Insulin-like growth factor (IGF) binding proteins (IGFBP) are expressed by multiple tissues and cells, including hepatic cells. By binding both IGF-1 and IGF-2, IGFBPs play a critical role in the regulation of IGF action, altering IGF interaction with cell surface receptors and increasing circulating IGF half-life and bioavailability. While their functions at the cellular level are not fully understood, studies have demonstrated the capacity to both stimulate and inhibit IGF-1 activity, depending on the system involved and the IGFBP studied. The ability of IGFBPs to regulate cell sensitivity by inhibiting IGF signaling may function in the control of tumor cell growth. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- IGFBP-1: Intra-assay <10% C Inter-assay <15%
- IGFBP-2: Intra-assay <10% CV Inter-assay <15%
- IGFBP-3: Intra-assay <10% CV Inter-assay <15%
- IGFBP-4: Intra-assay <10% CV Inter-assay <15%
- IGFBP-5: Intra-assay <10% CV Inter-assay <15%
- IGFBP-6: Intra-assay <10% CV Inter-assay <15%
- IGFBP-7: Intra-assay <10% CV Inter-assay <15%
|
| Panel Type | MAGNETIC Endocrine |
| Quality Level | MQ200
|
| Applications
|
| Application | The Human IGF Binding Protein Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 7 IGFBP protein biomarkers in human serum, plasma and cell culture samples. |
| Key Applications |
|
| Application Notes | • To prevent possible IGFBP proteolysis, it is recommended that a protease inhibitor cocktail solubion be added to each blood sample after blood collection.
• A 2-hour sample incubation may be used, however, for optimal sensitivity, use an overnight (16-18 hour) incubation.
• Serum and plasma samples require (recommend) a 1:25 dilution with Assay Buffer.
• Tissue culture supernatants may be run neat or appropriately diluted in control medium. Each well requires 25 μL of sample, standard or control. |
| Biological Information
|
| Specificity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel. |
| Species Reactivity |
|
| Analytes Available |
- IGFBP-1
- IGFBP-2
- IGFBP-3
- IGFBP-4
- IGFBP-5
- IGFBP-6
- IGFBP-7
|
| Assay Panel | Everything you need in a single kit. |
| Cross Reactivity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel. |
| Accuracy |
- IGFBP-1 102%
- IGFBP-2 90%
- IGFBP-3 95%
- IGFBP-4 97%
- IGFBP-5 79%
- IGFBP-6 105%
- IGFBP-7 98%
|
| Physicochemical Information
|
| Sensitivity |
- MinDC+2SD
- IGFBP-1: 0.01 ng/mL
- IGFBP-2: 1.09 ng/mL
- IGFBP-3: 0.15 ng/mL
- IGFBP-4: 0.96 ng/mL
- IGFBP-5: 9.87 ng/mL
- IGFBP-6: 0.12 ng/mL
- IGFBP-7: 0.07 ng/mL
|
| Linearity of Dilution | Contact technical support for this information |
| Standard Curve Range |
- IGFBP-1: 0.03 - 25 ng/mL
- IGFBP-2: 0.34 - 250 ng/mL
- IGFBP-3: 0.34 - 250 ng/mL
- IGFBP-4: 0.69 - 500 ng/mL
- IGFBP-5: 1.37 - 1000 ng/mL
- IGFBP-6: 0.14 - 100 ng/mL
- IGFBP-7: 0.07 - 50 ng/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
| Material Package | 96 well plate |
