millipore,
密理博,HGAMMAG-303E,MILLIPLEX MAP Human Immunoglobulin IgE Single Plex Magnetic Bead Kit - Isotyping Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| IgE
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | HGAMMAG-303E |
| Trade Name |
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| Description | MILLIPLEX® MAP Human Immunoglobulin IgE Single Plex Magnetic Bead Kit - Isotyping Assay |
| Background Information | Found only in mammals, Immunoglobulin E (IgE) plays an essential role in allergic reactions, and is predominantly associated with type 1 hypersensitivity. IgE is the least abundant immunoglobulin isotype in normal individuals, present at only 0.05% of IgG serum concentrations. It is capable of triggering robust immune reactions, with up to 10 times the typical level detected in the serum of atopic patients, although increased levels occur only in approximately 50% of patients with allergic disorders. IgE binds with high affinity to FcεRI, found
primarily on mast cells and basophils, priming the cells to recognize specific antigens such as dust mites, pet dander or pollen. Once primed and with repeated exposure to the same antigen, these cells release molecules such as histamines, prostaglandins and leukotrienes, resulting in an “allergic reaction”.
Although the mechanisms of this immune response are well understood, continued research is needed to understand the varying degrees of allergic responses – from mild rhinitis to anaphylaxis. Consequently, the MILLIPLEX MAP Human IgE Magnetic Bead Single Plex Kit enables you to measure levels of IgE in only 50 μL of 1:50 diluted human serum or plasma, saving both time and precious sample.
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| Product Information
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| Components |
- MILLIPLEX® MAP Anti-Human k and λ Light Chain, PE
- MILLIPLEX® MAP Human IgE Standard
- MILLIPLEX® MAP Human Serum Positive Control
- MILLIPLEX® MAP Assay Buffer
- MILLIPLEX® MAP Washer Buffer, 10X
- Set of one 96-Well Filter Plate with 4 Sealers
|
| Detection method | Luminex xMAP |
| Configuration | Single Plex |
| Precision, % |
- Intra-Assay < 10
- Inter-Assay < 13
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| Panel Type | MAGNETIC Immune Response |
| Quality Level | MQ200
|
| Applications
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| Application | Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in human serum, plasma and cell culture samples. |
| Key Applications |
|
| Application Notes | • This kit may be used for the analysis of IgE in human serum, plasma or tissue/cell lysate and culture supernatant samples.
• There are 2 one-hour room-temperature incubations.
• This assay requires 50 µl of 1:50 diluted plasma or serum, or 50 μL cell culture supernatant per well. |
| Biological Information
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| Specificity | Cross-reactivity between these antibodies and those against other immunoglobulins is non-detectable or negligible. |
| Species Reactivity |
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| Analytes Available |
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| Accuracy |
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| Physicochemical Information
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| Sensitivity |
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| Standard Curve Range |
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| Product Usage Statements
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| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| Storage and Shipping Information
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
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| Material Size | 96-well plate |
| Material Package | 96 Well Plate |
