millipore,密理博,HCYTOMAG-60K,MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay
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| Catalogue Number | HCYTOMAG-60K |
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| Description | MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay |
| Overview | To identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. The MILLIPLEX® MAP Human Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity. References: Mol Psychiatry. 2016 May 24. doi: 10.1038/mp.2016.77. Autism with intellectual disability is associated with increased levels of maternal cytokines and chemokines during gestation. Jones KL, Croen LA, Yoshida CK, Heuer L, Hansen R, Zerbo O, DeLorenze GN, Kharrazi M, Yolken R, Ashwood P, Van de Water J. Aust N Z J Psychiatry. 2016 Jun 16. pii: 0004867416652736. Inflammatory cytokines in major depressive disorder: A case-control study. Cassano P, Bui E, Rogers AH, Walton ZE, Ross R, Zeng M, Nadal-Vicens M, Mischoulon D, Baker AW, Keshaviah A, Worthington J, Hoge EA, Alpert J, Fava M, Wong KK, Simon NM. PLoS One. 2016 Feb 26;11(2):e0149411. doi: 10.1371/journal.pone.0149411. eCollection 2016. B Cell, Th17, and Neutrophil Related Cerebrospinal Fluid Cytokine/Chemokines Are Elevated in MOG Antibody Associated Demyelination. Kothur K, Wienholt L, Tantsis EM, Earl J, Bandodkar S, Prelog K, Tea F, Ramanathan S, Brilot F, Dale RC. Transl Psychiatry. 2016 May 31;6(5):e822. doi: 10.1038/tp.2016.91. Prenatal immune programming of the sex-dependent risk for major depression. Gilman SE, Cherkerzian S, Buka SL, Hahn J, Hornig M, Goldstein JM. |
| Background Information | “Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. Also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer. |
| Product Information | |
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| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
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| Multiplex Ordering Requirements | Please note: Sample Type must be selected when configuring this kit. RANTES, PDGF-AA and PDGF-AB/AA cannot be plexed with other analytes for serum/plasma. |
| Panel Type | MAGNETIC Cytokines/Chemokines |
| Quality Level | MQ200 |
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| Application | Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples. |
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| Application Notes | • This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended. • This assay requires 25 µl of neat plasma or serum, or 25 μL cell culture supernatant per well. • This kit may be used for the analysis of all or any combination of the cytokines and chemokines in serum, plasma or tissue/cell lysate and culture supernatant samples with the exception of RANTES, PDGF-AA and PDGF-AB/BB. • RANTES, PDGF-AA and PDGF-AB/BB cannot be plexed with other cytokines in this panel due to a required 1:100 dilution of plasma/serum samples. |
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| Cross Reactivity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information | |
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| Material Size | 96-well plate |
| Material Package | 96 well plate |
