millipore,
密理博,HLPPMAG-57K,MILLIPLEX MAP Human Liver Protein Magnetic Bead Panel - Metabolism Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| AFP, ANGPTL3, ANGPTL4, ANGPTL6/AGF, FABP1, FGF-19, FGF-21, FGF-23, HGF
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | HLPPMAG-57K |
| Trade Name |
|
| Description | MILLIPLEX MAP Human Liver Protein Magnetic Bead Panel - Metabolism Multiplex Assay |
| Overview | millipore’s MILLIPLEX MAP Human Liver Protein Panel is a 9-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue/cell culture samples: AFP, ANGPTL3, ANGPTL4, ANGPTL6/AGF, FABP1/L-FABP, FGF-19, FGF-21, FGF-23 and HGF.
NOTE: When assaying ANGPTL6/AGF it is recommended that serum samples be used. |
| Background Information | Liver-secreted proteins play important roles in metabolic regulation. For example, liver-secreted proteins have been shown to regulate circulating lipoprotein levels, energy expenditure, glucose metabolism, and fatty acid uptake. In addition, some liver-secreted proteins may also serve as biomarkers for liver diseases and gastric cancer. Accurate measurement of liver proteins is critical to obtain understanding of their biological functions. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- AFP: Intra-assay <10% CV Inter-assay <20%
- ANGPTL3: Intra-assay <10% CV Inter-assay <20%
- ANGPTL4: Intra-assay <10% CV Inter-assay <20%
- ANGPTL6/AGF: Intra-assay <10% CV Inter-assay <20%
- FABP1: Intra-assay <10% CV Inter-assay <20%
- FGF-19: Intra-assay <10% CV Inter-assay <20%
- FGF-21: Intra-assay <10% CV Inter-assay <20%
- FGF-23: Intra-assay <10% CV Inter-assay <20%
- HGF: Intra-assay <10% CV Inter-assay <20%
|
| Panel Type | MAGNETIC Metabolism |
| Quality Level | MQ200
|
| Applications
|
| Application | The Human Liver Protein Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 9 liver protein biomarkers in human serum, plasma and tissue/cell culture samples. |
| Key Applications |
|
| Application Notes | A 2 hour sample incubaton may be used, however, for optimal sensitivity, use an overnight (16-18 hour) incubation. Serum and plasma samples require (recommend) a 1:2 dilution with Assay Buffer. Tissue culture supernatants may be run neat or appropriately diluted in control medium. Each well requires 25 μL of sample, standard or control. |
| Biological Information
|
| Specificity | The ANGPTL6 standard had cross reactivity with the ANGPTL4 assay of less than 2%. For all other analytes, there was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel. |
| Species Reactivity |
|
| Analytes Available |
- AFP
- ANGPTL3
- ANGPTL4
- ANGPTL6/AGF
- FABP1
- FGF-19
- FGF-21
- FGF-23
- HGF
|
| Assay Panel | Everything you need in a single kit. |
| Cross Reactivity | The ANGPTL6 standard had cross reactivity with the ANGPTL4 assay of less than 2%. For all other analytes, there was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel. |
| Accuracy |
- AFP 94.2%
- ANGPTL3 93.7%
- ANGPTL4 96.8%
- ANGPTL6 99.1%
- FABP1 96.6%
- FGF-19 98.0%
- FGF-21 98.8%
- FGF-23 96.6%
- HGF 100.2%
|
| Physicochemical Information
|
| Sensitivity |
- MinDC+2SD
- AFP: 0.144 ng/mL
- ANGPTL3: 0.725 ng/mL
- ANGPTL4: 2.150 ng/mL
- ANGPTL6: 0.847 ng/mL
- FABP1: 0.218 ng/mL
- FGF-19: 0.054 ng/mL
- FGF-21: 0.004 ng/mL
- FGF-23: 0.014 ng/mL
- HGF: 0.018 ng/mL
|
| Linearity of Dilution | Contact technical support for this information |
| Standard Curve Range |
- AFP: 0.14 - 100 ng/mL
- ANGPTL3: 0.69 - 500 ng/mL
- ANGPTL4: 5.49 - 4000 ng/mL
- ANGPTL6/AGF: 1.37 - 1000 ng/mL
- FABP1: 0.27 - 200 ng/mL
- FGF-19: 0.14 - 100 ng/mL
- FGF-21: 0.01 - 10 ng/mL
- FGF-23: 0.04 - 30 ng/mL
- HGF: 0.07 - 50 ng/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
| Material Package | 96 well plate |
