millipore,
密理博,HNABTMAG-68K,MILLIPLEX MAP Human Amyloid Beta and Tau Magnetic Bead Panel - Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| Tau (Total), Amyloid β40, Amyloid β42, Tau (pThr181)
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | HNABTMAG-68K |
| Trade Name |
|
| Description | MILLIPLEX MAP Human Amyloid Beta and Tau Magnetic Bead Panel - Multiplex Assay |
| Overview | The MILLIPLEX® MAP Human Amyloid Beta Tau Magnetic BeadPanel is to be used for the simultaneous quantification of Aβ40, Aβ42, tTau, and pTau181. This kit may be used only for the analysis of cerebrospinal fluid (CSF). This is a configurable assay, and some or all analytes can be selected: tau (total), amyloid β40, amyloid β42 and tau (pThr181).
MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.
Each MILLIPLEX® MAP panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment
• Detection antibody cocktails designed to yield consistent analyte profiles within panel
EMD millipore’s MILLIPLEX® MAP Human Amyloid Beta Tau Magnetic Bead Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation. |
| Background Information | The MILLIPLEX® MAP Human Amyloid Beta and Tau Panel enables you to focus on the relevance and therapeutic potential of Aβ40, Aβ42, Total Tau proteins (tTau), and Phosphorylated Tau Thr181 (pTau181) in your research model. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity. |
| Product Information
|
| Components |
- Human ABeta and Tau Standard, 1 vial, lyophilized
- Human ABeta and Tau Quality Control 1, 1 vial, lyophilized
- Human ABeta and Tau Quality Control 2, 1 vial, lyophilized
- Bead Diluent, 1 bottle, 3.5mL
- Set of one 96-Well Plate with 2 sealers
- Assay Buffer, 1 bottle, 30 mL
- Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
- Human ABeta and Tau Detection Antibodies, 1 bottle, 3.2 mL
- Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL
- Mixing Bottle, 1 bottle
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- Inter-assay: < 15% CV
- Intra-assay: < 10% CV
|
| Panel Type | MAGNETIC Neuroscience |
| Quality Level | MQ200
|
| Applications
|
| Application | The MILLIPLEX® MAP Human Amyloid Beta and Tau Panel is a 4-plex kit containing all reagents needed for simultaneous quantification of Aβ40, Aβ42, Total Tau proteins (tTau), and Phosphorylated Tau Thr181 (pTau181) in CSF samples. |
| Key Applications |
|
| Application Notes | EMD millipore’s MILLIPLEX® MAP Human Amyloid Beta and Tau Panel is a 4-plex kit used for the simultaneous quantification of any or all of the following analytes in cerebrospinal fluid (CSF) samples: Amyloid beta 1-40 (Aβ40), Amyloid beta 1-42 (Aβ42), Total Tau proteins (tTau), and Phosphorylated Tau Thr181 (pTau181).
• This is a competitive assay. An overnight (16-18 hours) incubation at 20-25°C is recommended.
• This assay requires 25 μL of diluted cerebrospinal fluid (CSF).
• A 1:2 sample dilution is recommended for extracted CSF samples. |
| Biological Information
|
| Specificity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. |
| Species Reactivity |
|
| Analytes Available |
- Tau (Total)
- Amyloid β40
- Amyloid β42
- Tau (pThr181)
|
| Assay Panel | Everything you need in a single kit. |
| Cross Reactivity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. |
| Accuracy |
- Spike recovery for Aβ1-40 is 101%.
- Spike recovery for Aβ1-42 is 109%.
- Spike recovery for tTau is 98%.
- Spike recovery for pTau181 is 91%
|
| Physicochemical Information
|
| Sensitivity |
- Aβ1-40: 10.2 pg/mL (MinDC+2SD)
- Aβ1-42: 2.5 pg/mL (MinDC+2SD)
- tTau: 14.2 pg/mL (MinDC+2SD)
- pTau181: 1.5 pg/mL (MinDC+2SD)
|
| Standard Curve Range |
- Aβ1-40: 21-15000 pg/mL
- Aβ1-42: 2-2000 pg/mL
- tTau: 11-8000 pg/mL
- pTau181: 0.7-500 pg/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
| Material Package | 96-well plate |
