| Appearance (clearness) | clear |
| Appearance (color) | darkblue |
| pH-value (25 °C) | 5.0 - 5.4 |
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Salmonella, E.coli and P.aeruginosa
Additionaly monoculture of E.coli and E.faecalis
Confirmation on XLD agar resp. Tryptic soy agar |
| Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)) | ≤ 100 |
| Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)) | ≤ 100 |
| Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)) | ≥ 10000 |
| Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)) | ≥ 10000 |
| Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)) | ≥ 10000 |
| Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)) | ≥ 10000 |
| Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)) | ≥ 10000 |
| Growth on XLD agar (Salmonella typhimurium ATCC 14028 (WDCM 00031)) | > 10 colonies |
| Growth on XLD agar (Salmonella enteritidis ATCC 13076 (WDCM 00030)) | > 10 colonies |
| Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031)) | colourless with black centre |
| Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030)) | colourless with black centre |
| Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)) | < 100 colonies |
| Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)) | < 100 colonies |
| Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)) | < 10 colonies |
| Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)) | < 10 colonies |
Incubation:
RVS broth: 24 ± 3 hours at 41.5 ± 1 °C aerobic
Confirmation on XLD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobic
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