millipore,
密理博,MGAMMAG-300K,MILLIPLEX MAP Mouse Immunoglobulin Isotyping Magnetic Bead Panel - Isotyping Multiplex Assay
| Replacement Information
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| Replacement Information | This product replaces Mouse Immunoglobulin Isotyping Kit, MGAM-300 in December of 2011 or while supplies last. |
| Description
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| Catalogue Number | MGAMMAG-300K |
| Replaces | MGAM-300 |
| Trade Name |
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| Description | MILLIPLEX MAP Mouse Immunoglobulin Isotyping Magnetic Bead Panel - Isotyping Multiplex Assay |
| Overview | MILLIPLEX MAP Mouse Isotyping Magnetic Bead Panel, measuring mouse IgG subclasses (1, 2a, 2b, 3), IgM, and IgA, has been designed to enable you to measure both classes and subclasses accurately – all in one well. In addition this panel allows you to choose only those immunoglobulins you need for your experiments. |
| Background Information | Produced by plasma cells and lymphocytes, immunoglobulins (antibodies) are critically involved in immune response, attaching to antigens and playing a role in their destruction. Immunoglobulins (Ig) can be classified by isotype, classes that differ in function and antigen response due to structure variability. Six major isotypes have been identified in placental mammals: IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA – all found in normal individuals. Immunoglobulin-deficiency disorders, such as autoimmune disease, some GI conditions and malignancies, are characterized by specific isotype deficiencies or varying concentrations of one or more isotypes. Disease states can range from the absence of one isotype class or subclass to a total deficiency of immunoglobulin classes. |
| Product Information
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| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- Intra-Assay 5 - 10%
- Inter-Assay 14 - 17%
|
| Panel Type | MAGNETIC Immune Response |
| Quality Level | MQ200
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| Applications
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| Application | Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in murine serum, plasma and cell culture samples. |
| Key Applications |
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| Application Notes | • This kit may be used for the analysis of IgG1, IgG2a, IgG2b, IgG3, IgA and IgM in mouse serum or tissue/cell lysate and culture supernatant samples.
• There are 2 one-hour room-temperature incubations.
• This assay requires 50 µl of 1:50,000 diluted plasma or serum, or 50 μL cell culture supernatant diluted to approximately 1μg/mL Ig per well. |
| Biological Information
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| Specificity | Cross-reactivity between these antibodies and those against other immunoglobulins is non-detectable or negligible. |
| Species Reactivity |
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| Analytes Available |
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| Accuracy |
- IgG1 96%
- IgG2a 76%
- IgG2b 98%
- IgG3 85%
- IgA 98%
- IgM 100%
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| Physicochemical Information
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| Sensitivity |
- IgG1 0.7 ng/mL
- IgG2a 0.67 ng/mL
- IgG2b 0.18 ng/mL
- IgG3 0.32 ng/mL
- IgA 1.1 ng/mL
- IgM 1.73 ng/mL
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| Standard Curve Range |
- IgG1: 2 – 1,500 ng/mL
- IgG2a: 3 – 2,000 ng/mL
- IgG2b: 1 – 900 ng/mL
- IgG3: 2 – 1,500 ng/mL
- IgA: 5 – 3,000 ng/mL
- IgM: 2 – 1,500 ng/mL
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| Product Usage Statements
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| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| Storage and Shipping Information
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
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| Material Size | 96-well plate |
| Material Package | 96 Well Plate |
