millipore,
密理博,MHSTCMAG-70K,MILLIPLEX MAP Mouse High Sensitivity T Cell Panel - Immunology Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 (p70), IL-13, IL-17A, KC/CXCL1, LIX, MCP-1, MIP-2, TNF-α
| M
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | MHSTCMAG-70K |
| Trade Name |
|
| Description | MILLIPLEX MAP Mouse High Sensitivity T Cell Panel - Immunology Multiplex Assay |
| Background Information | Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells), and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells, and myeloid cells. Research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.
Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- Inter-assay: <15%
- Intra-assay: <10%
|
| Panel Type | MAGNETIC Cytokines/Chemokines |
| Quality Level | MQ200
|
| Applications
|
| Application | Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples. |
| Key Applications |
|
| Application Notes | • This is an overnight incubation assay.
• A maximum of 25 μL per well of neat or1:2 diluted serum or plasma can be used. Tissue culture or other media may also be used. |
| Biological Information
|
| Specificity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Species Reactivity |
|
| Analytes Available |
- GM-CSF
- IFNγ
- IL-1α
- IL-1β
- IL-2
- IL-4
- IL-5
- IL-6
- IL-7
- IL-10
- IL-12 (p70)
- IL-13
- IL-17A
- KC/CXCL1
- LIX
- MCP-1
- MIP-2
- TNF-α
|
| Cross Reactivity | There is no detectable cross-reactivity within the panel. |
| Accuracy |
|
| Physicochemical Information
|
| Sensitivity |
- 0.26 - 11.14 pg/mL
- Please see protocol for sensitivity of individual analytes.
|
| Linearity of Dilution | |
| Standard Curve Range |
- GM-CSF: 6.1 – 25,000 pg/mL
- IFNγ: 0.49 – 2,000 pg/mL
- IL-1α: 3.91 – 16,000 pg/mL
- IL-1β: 3.91 – 16,000 pg/mL
- IL-2: 0.98 – 4,000 pg/mL
- IL-4: 0.10 – 400 pg/mL
- IL-5: 0.98 – 4,000 pg/mL
- IL-6: 0.98 – 4,000 pg/mL
- IL-7: 0.49 – 2,000 pg/mL
- IL-10: 1.34 – 5,500 pg/mL
- IL-12 (p70): 2.93 – 12,000 pg/mL
- IL-13: 3.42 – 14.000 pg/mL
- IL-17A: 0.49 – 2,000 pg/mL
- KC/GROα/CXCL1: 0.73 – 3,000 pg/mL
- LIX: 3.17 – 13,000 pg/mL
- MCP-1/CCL2: 3.91 – 16,000 pg/mL
- MIP-2/CXCL2: 7.32 – 30,000 pg/mL
- TNFα: 0.49 – 2,000 pg/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
