millipore,
密理博,MKI1MAG-94K,MILLIPLEX MAP Mouse Kidney Injury Magnetic Bead Panel 1 - Toxicity Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| β-2-Microglobulin, IP-10, KIM-1, Renin, TIMP-1, VEGF
| M
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | MKI1MAG-94K |
| Trade Name |
|
| Description | MILLIPLEX MAP Mouse Kidney Injury Magnetic Bead Panel 1 - Toxicity Multiplex Assay |
| Overview | The MILLIPLEX® MAP Mouse Kidney Injury Magnetic Bead Panel 1 contains all the components necessary to measure the following six biomarkers in any combination using Luminex® xMAP® technology: β-2-Microglobulin, IP-10, KIM-1, Renin, TIMP-1, VEGF. The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 urine samples in duplicate. |
| Background Information | Maintenance of physiological balance in the body is attributed in part to normal kidney function. An altered kidney function due to injury, drug toxicity or kidney failure may be life threatening. There are multiple risk factors for kidney injury including age, hypertension, diabetes mellitus, heart failure and liver failure. On the other hand, drug induced damage to the kidney results from drug excretion. Traditional markers for assessing renal toxicity, such as blood urea nitrogen (BUN) and serum creatinine, are insensitive and non-specific. Although both are direct measurements of renal function, increases in serum concentrations of these biomarkers occur only after substantial renal injury. Specificity and earlier detection of kidney injury is vital. For improved detection of acute nephrotoxicity, a panel of novel urinary kidney biomarkers has been identified and validated for preclinical studies in relevant animal species.
We provide valuable research assays to investigate multiple biomarkers of kidney injury in mouse urine samples using the Luminex xMAP® instrument platform. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- Inter-assay: < 10
- Intra-Assay: < 10
|
| Panel Type | MAGNETIC Toxicity |
| Quality Level | MQ200
|
| Applications
|
| Application | The analytes available for this multiplex kit are: β-2-Microglobulin, IP-10, KIM-1, Renin, TIMP-1, VEGF (for urine samples) or IP-10, KIM-1, Renin, and TIMP-1 (for serum/plasma samples). |
| Key Applications |
|
| Application Notes | • This is an overnight assay.
• This assay requires 25 µL of 1:25 diluted urine sample per well in kit Assay Buffer (please dilute per protocol instructions). |
| Biological Information
|
| Specificity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. |
| Species Reactivity |
|
| Analytes Available |
- β-2-Microglobulin
- IP-10
- KIM-1
- Renin
- TIMP-1
- VEGF
|
| Assay Panel | Everything you need in a single kit. |
| Cross Reactivity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. |
| Accuracy |
|
| Physicochemical Information
|
| Sensitivity |
- 0.001 - 0.041 ng/mL (MinDC+2SD).
- Please see kit protocol for individual assay sensitivities.
|
| Linearity of Dilution | Contact technical support for this information. |
| Standard Curve Range |
- β-2-Microglobulin: 0.05 – 50 ng/mL
- IP-10: 0.005 – 5 ng/mL
- KIM-1: 0.01 – 15 ng/mL
- Renin: 0.05 – 50 ng/mL
- TIMP-1: 0.02 – 25 ng/mL
- VEGF: 0.001 – 1 ng/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
