millipore,
密理博,MGAMMAG-300E,MILLIPLEX MAP Mouse IgE Single Plex Magnetic Bead Kit - Isotyping Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| IgE
| M
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | MGAMMAG-300E |
| Trade Name |
|
| Description | MILLIPLEX® MAP Mouse IgE Single Plex Magnetic Bead Kit - Isotyping Multiplex Assay |
| Background Information | Found only in mammals, Immunoglobulin E (IgE) plays an essential role in allergic reactions, and is predominantly associated with type 1 hypersensitivity. IgE is the least abundant immunoglobulin isotype in normal or topic mammals, present at only 0.05% of IgG serum concentrations. It is capable of triggering robust immune reactions, with up to 10 times the typical level detected in the serum of atopic mammals, although increased levels occur only in approximately 50% of mammals with allergic disorders. IgE binds with high affinity to FcɛRI,
found primarily on mast cells and basophils, priming the cells to recognize specific antigens. Once primed and with repeated exposure to the same antigen, these cells release molecules such as histamines, prostaglandins and
leukotrienes, resulting in an “allergic reaction”. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Single Plex |
| Precision, % |
- Intra-Assay < 5%
- Inter-Assay < 10%
|
| Panel Type | MAGNETIC Immune Response |
| Quality Level | MQ200
|
| Applications
|
| Application | Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulin E (IgE) in mouse serum, plasma and cell culture samples. |
| Key Applications |
|
| Application Notes | • This kit may be used for the analysis of Mouse IgE in Mouse serum, plasma, tissue/cell lysates and tissue culture supernatant samples.
• The assay can be performed in less than 3 hours
• This assay utilizes as little as 2uL of sample (a 1:200 dilution for plasma or serum is recommended; 2uL in 398uL of Assay Buffer). Tissue/ cell culture supernatants are diluted to approximately 1μg/mL in Assay Buffer |
| Biological Information
|
| Specificity | Cross-reactivity between these antibodies and those against other immunoglobulins is non-detectable or negligible. |
| Species Reactivity |
|
| Analytes Available |
|
| Accuracy |
|
| Physicochemical Information
|
| Sensitivity |
|
| Standard Curve Range |
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| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
| Material Package | 96 Well Plate |
