millipore,
密理博,MECY2MAG-73K,MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel II, MECY2MAG-73K
| Description
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| Catalogue Number | MECY2MAG-73K |
| Trade Name |
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| Description | MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel II, MECY2MAG-73K |
| Overview | The MILLIPLEX® MAP Mouse Cytokine/Chemokine Magnetic Bead Panel II, contains all the components necessary to measure the following 15 biomarkers in any combination using Luminex® xMAP® technology: Erythropoietin (EPO), Exodus-2/CCL21/6Ckine, Fractalkine/CX3CL1, IFNβ1, IFNγ, IL-11, IL-16, IL-17A/F, IL-20, MCP-5/CCL12, MDC/CCL22, MIP-3α/CCL20, MIP-3β/CCL19, TARC /CCL17, TIMP-1. The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 serum, plasma, or cell/tissue culture samples in duplicate. |
| Background Information | Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. In addition the mouse model and mouse-to-human translational studies provide data essential to understanding the role of cytokines and immune response in both normal and disease states. |
| Product Information
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| Components |
- Mouse Cytokine/Chemokine Panel II Standard, 1 vial, lyophilized
- Mouse Cytokine/Chemokine Panel II Quality Control 1, 1 vial, lyophilized
- Mouse Cytokine/Chemokine Panel II Quality Control 2, 1 vial, lyophilized
- Serum Matrix (0.08% Sodium Azide), 1 vial, lyophylized
- Assay Buffer, 1 bottle, 30 mL
- Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
- Mouse Cytokine/Chemokine Panel II Detection Antibodies, 1 bottle, 3.2 mL
- Mouse Cytokine/Chemokine Panel II Antibody-Immobilized Beads (50X), 90 µL ea
- Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL
- Mixing Bottle, 1 bottle
- Sample Plate, 96 well format, 1 plate
- 2 Foil Plate Sealers
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| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- Inter-assay: <20
- Intra-assay: <10
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| Panel Type | MAGNETIC Cytokines/Chemokines |
| Quality Level | MQ200
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| Applications
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| Application | Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples. |
| Key Applications |
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| Application Notes | This assay is an overnight assay (16 hour incubation) or same day assay (2 hour incubation). Serum and plasma samples require (recommend) a 1:2 dilution with Assay Buffer. Each well requires 25 μL of sample, standard or control. Tissue culture supernatants can also be used and if needed, make appropriate dilutions with control medium. |
| Biological Information
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| Specificity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. |
| Species Reactivity |
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| Analytes Available |
- Erythropoietin (EPO)
- Exodus-2
- Fractalkine/CX3CL1
- IFNβ1
- IFNγ
- IL-11
- IL-16
- IL-17A/F
- IL-20
- MCP-5/CCL12
- MDC (CCL22)
- MIP-3α/CCL20
- MIP-3β/CCL19
- TARC/CCL17
- TIMP-1
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| Assay Panel | Everything you need in a single kit. |
| Cross Reactivity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. |
| Accuracy |
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| Physicochemical Information
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| Sensitivity |
- Refer to kit protocol for sensitivities of individual cytokines/chemokines.
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| Linearity of Dilution | Contact technical support for this information. |
| Standard Curve Range |
- Erythropoietin (EPO): 5 – 20,000 pg/mL
- Exodus-2/CCL21/6Ckine: 12 – 50,000 pg/mL
- Fractalkine/CX3CL1: 24 – 100,000 pg/mL
- IFNβ1: 12 – 50,000 pg/mL
- IFNγ: 1 – 5,000 pg/mL
- IL-11: 12 – 50,000 pg/mL
- IL-16: 2 – 10,000 pg/mL
- IL-17A/F: 24 – 100,000 pg/mL
- IL-20: 12 – 50,000 pg/mL
- MCP-5/CCL12: 2 – 10,000 pg/mL
- MDC/CCL22: 2 – 10,000 pg/mL
- MIP-3α/CCL20: 2 – 10,000 pg/mL
- MIP-3β/CCL19: 12 – 50,000 pg/mL
- TARC/CCL17: 2 – 10,000 pg/mL
- TIMP-1: 2 – 10,000 pg/mL
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| Product Usage Statements
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| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| Storage and Shipping Information
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
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| Material Size | 96-well plate |
