millipore,密理博,MCYTOMAG-70K,MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay
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| Catalogue Number | MCYTOMAG-70K |
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| Description | MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay |
| Overview | To identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include: • More flexible plate and plate washer options • Improved performance with turbid serum/plasma samples • Assay results equivalent to non-magnetic beads • Automated magnetic washing avoids many problems associated with vacuum filtration washing MILLIPLEX MAP Mouse Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity. References: Neuroscience. 2015 Sep 24;304:316-27. doi: 10.1016/j.neuroscience.2015.07.070. Nilotinib and bosutinib modulate pre-plaque alterations of blood immune markers and neuro-inflammation in Alzheimer's disease models. Lonskaya I, Hebron ML, Selby ST, Turner RS, Moussa CE. Mol Neurodegener. 2016 Apr 2;11:25. doi: 10.1186/s13024-016-0090-7. Pioglitazone ameliorates the phenotype of a novel Parkinson's disease mouse model by reducing neuroinflammation. Pinto M, Nissanka N, Peralta S, Brambilla R, Diaz F, Moraes CT. Sci Rep. 2016 May 13;6:25663. doi: 10.1038/srep25663. CSF1R blockade slows the progression of amyotrophic lateral sclerosis by reducing microgliosis and invasion of macrophages into peripheral nerves. Martínez-Muriana A, Mancuso R, Francos-Quijorna I, Olmos-Alonso A, Osta R, Perry VH, Navarro X, Gomez-Nicola D, López-Vales R. Neuropharmacology. 2016 Apr 20. pii: S0028-3908(16)30161-7. doi: 10.1016/j.neuropharm.2016.04.021. Ketamine modulates hippocampal neurogenesis and pro-inflammatory cytokines but not stressor induced neurochemical changes. Clarke M, Razmjou S, Prowse N, Dwyer Z, Litteljohn D, Pentz R, Anisman H, Hayley S. Front Cell Neurosci. 2016 Jun 28;10:169. doi: 10.3389/fncel.2016.00169. eCollection 2016. Allergy Enhances Neurogenesis and Modulates Microglial Activation in the Hippocampus. Klein B, Mrowetz H, Thalhamer J, Scheiblhofer S, Weiss R, Aigner L. Glia. 2016 Sep;64(9):1479-91. doi: 10.1002/glia.23016. Microglial phagocytosis and activation underlying photoreceptor degeneration is regulated by CX3CL1-CX3CR1 signaling in a mouse model of retinitis pigmentosa. Zabel MK, Zhao L, Zhang Y, Gonzalez SR, Ma W, Wang X, Fariss RN, Wong WT. J Immunol. 2016 Apr 15;196(8):3318-30. doi: 10.4049/jimmunol.1502021. Age-Associated Resident Memory CD8 T Cells in the Central Nervous System Are Primed To Potentiate Inflammation after Ischemic Brain Injury. Ritzel RM, Crapser J, Patel AR, Verma R, Grenier JM, Chauhan A, Jellison ER, McCullough LD. Mol Psychiatry. 2016 Jul;21(7):956-68. doi: 10.1038/mp.2015.222. Maternal immune activation leads to selective functional deficits in offspring parvalbumin interneurons. Canetta S, Bolkan S, Padilla-Coreano N, Song LJ, Sahn R, Harrison NL, Gordon JA, Brown A, Kellendonk C. J Mol Cell Cardiol. 2017 Jun;107:22-26. doi: 10.1016/j.yjmcc.2017.04.003. A humanized HLA-DR4 mouse model for autoimmune myocarditis. Selli ME, Thomas AC, Wraith DC, Newby AC. |
| Background Information | The immune system is an organized complex network of biological structures and processes that protect against disease. For example, cytokine and chemokines mediate interactions between cells directly, regulating target immune cell responses. These responses result in inflammation where the immune system attempts to eradicate foreign antigens and begin the healing process. Consequently, the inflammatory process plays a key protective role in immunity. In addition cytokine and chemokine research is essential in understanding the immune system and its multi-faceted response to most antigens, as well as disease states such as autoimmune disease, allergic reactions, sepsis and cancer. |
| Product Information | |
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| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
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| Panel Type | MAGNETIC Cytokines/Chemokines |
| Quality Level | MQ200 |
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| Application | Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples. |
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| Application Notes | • This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended. • This assay requires 25 µl of 1:2 diluted plasma or serum, or 25 μL cell culture supernatant per well. |
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| Cross Reactivity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information | |
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| Material Size | 96-well plate |
| Material Package | 96 well plate |
