重要规格表
| Species Reactivity | Key Applications |
|---|---|
| R, M, NhP, Fe, Ca | Single Molecule Counting (SMC™) |
| Description | |
|---|---|
| Catalogue Number | 03-0181-00 |
| Trade Name |
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| Description | SMC™ Human IFN-γ High Sensitivity Immunoassay Kit |
| Overview | The SMC™ Human IFN-γ High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna®and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | Interferon gamma is a pro-inflammatory cytokine produced by activated T lymphocytes and natural killer cells. It is important in the activation of macrophages and MHC expression and plays a key role in both inflammation and autoimmune disease by enhancing antigen presentation, increasing leukocyte trafficking and affecting cell proliferation and apoptosis. |
| Product Information | |
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| Components |
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| Precision, % |
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| Applications | |
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| Application | The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IFN-γ High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IFN-γ in human serum and plasma. |
| Key Applications |
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| Application Notes | This new streamline kit has been improved with the following features over the legacy IFN-γ kit, #03-0049-00: • LLOQ has been lowered from 0.2 pg/mL in the legacy kit to 0.03 pg/mL in the new kit • Improved options for sample prep and incubation • Lyophilization of the standard provides the kit at one storage condition • Removal of a plate transfer step • Increased reagent volumes to accommodate automation • Validated to run on both SMCxPRO™ and Erenna® • Tested against WHO standard Note: Recombinant protein is not stable and vortexing is not recommended. |
| Biological Information | |
|---|---|
| Species Reactivity |
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| Species Reactivity Note | Although IFN-γ was detectable in rat, mouse, non-human primate, feline, and canine plasma, the assay has not been validated for these species. |
| Cross Reactivity | The SMC™ Human IFN-γ High Sensitivity Immunoasay Kit was not found to be cross-reactive with the following: rhIFN-α2 rhIFN-β rhIFN-λ1 (IL-29) rhIFN-λ2 (IL-28A) rhIFN-λ3 (IL-28B) rhIFN-ω2 |
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| Physicochemical Information | |
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| Product Usage Statements | |
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| Packaging Information | |
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| Material Size | 96 well assay |
