millipore,
密理博,03-0176-00,SMC Human IL-10 High Sensitivity Immunoassay Kit
重要规格表
| Species Reactivity | Key Applications |
|---|
| NhP, M, R, Ca, Fe
| Single Molecule Counting (SMC™)
|
| Description
|
| Catalogue Number | 03-0176-00 |
| Trade Name |
|
| Description | SMC™ Human IL-10 High Sensitivity Immunoassay Kit |
| Overview | The SMC™ Human IL-10 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | Interleukin 10 (IL-10) is an anti-inflammatory cytokine derived from T cells, mononuclear phagocytes and keratinocytes with effects on immunoregulation and inflammation. It has shown to inhibit the synthesis of pro-inflammatory cytokines while also suppressing the antigen-presentation capacity of antigen presenting cells. As well, IL-10 stimulates T cells and mast cells as well as B cell maturation and antibody production. |
| Product Information
|
| Components |
- Assay Buffer
- IL-10 Coated Beads
- Standard Diluent
- IL-10 Detection Antibody
- IL-10 Standard
- 10X Wash Buffer
- Buffer D
- Elution Buffer B
|
| Precision, % |
- Intra-assay CV% average (n=20): <15%
- Inter-assay CV% average (n=5): <20%
|
| Applications
|
| Application | The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-10 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-10 in human serum and plasma. |
| Key Applications |
- Single Molecule Counting (SMC™)
|
| Application Notes | This new streamline kit has been improved with the following features including new including new improvements over the legacy IL-10 kit, #03-0056-00:
• Improved LLOQ--0.10 pg/mL in the new streamlined kit while the legacy kit is 0.39 pg/mL
• Adjusted standard curve--changed to 0.1 – 100 pg/mL from 0.17 – 400 pg/mL
• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Removal of a plate transfer step
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO™ and Erenna® |
| Biological Information
|
| Species Reactivity |
- Non-human Primate
- Mouse
- Rat
- Canine
- Feline
|
| Species Reactivity Note | Although IL-10 was detectable in non-human primate, mouse, rat, canine, and feline plasma, the assay has not been validated for these species. |
| Accuracy |
- Minimum of 5 serum samples spiked with average recovery of 87% with a range of 84-93%
- Minimum of 5 plasma samples spiked with average recovery of 90% with a range of 82-97%
|
| Physicochemical Information
|
| Sensitivity |
- Lower Limit of Quantification: 0.10 pg/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| Packaging Information
|
| Material Size | 96 well assay |
