重要规格表
| Species Reactivity | Key Applications |
|---|---|
| NhP, Fe, Ca | Single Molecule Counting (SMC™) |
| Description | |
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| Catalogue Number | 03-0180-00 |
| Trade Name |
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| Description | SMC™ Human VEGF-A High Sensitivity Immunoassay Kit |
| Overview | The SMC™ Human VEGF-A High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | Vascular Endothelial Cell Growth Factor A (VEGF-A), one of five members of the VEGF family (VEGF-A, VEGF-B, VEGF-C, VEGF-D and Placenta Growth Factor), is a signaling protein critical in angiogenesis and vasculogenesis, predominantly acting on vascular endothelial cells. It has an important role in embryonic development and in wound healing in adults but overexpression in disease can lead to conditions such as vascular disease in the retina, tumor development and growth due to enhanced blood supply while a decrease in VEGF in pulmonary arteries is associated with pulmonary emphysema. |
| Product Information | |
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| Application | The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human VEGF-A High Sensitivity Immunoassay Kit unparalleled performance in the quantification of VEGF-A in human serum and plasma. |
| Key Applications |
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| Application Notes | This new streamline kit has been improved with the following features including new including new improvements over the legacy VEGF kit, #03-0068-00: • Improved options for sample prep and incubation • Lyophilization of the standard provides the kit at one storage condition • Removal of a plate transfer step • Increased reagent volumes to accommodate automation • Validated to run on both SMCxPRO™ and Erenna® |
| Biological Information | |
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| Species Reactivity |
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| Species Reactivity Note | Although VEGF was detectable in non-human primate, feline, and canine plasma, the assay has not been validated for these species. |
| Cross Reactivity | The SMC™ Human VEGF-A High Sensitivity Immunoasay Kit was not found to be cross-reactive with the following: rhVEGF-B rhVEGF-C rhVEGF-D |
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| Physicochemical Information | |
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| Packaging Information | |
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| Material Size | 96 well assay |
