重要规格表
| Species Reactivity | Key Applications |
|---|---|
| R, M, Mk, Ca, Fe | Single Molecule Counting (SMC™) |
| Description | |
|---|---|
| Catalogue Number | 03-0182-00 |
| Trade Name |
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| Description | SMC™ Human IL-12p70 High Sensitivity Immunoassay Kit |
| Overview | The SMC™ Human IL-12p70 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | Interleukin 12p70 (IL-12p70), belongs to the IL-12 family of cytokine responsible for immune response through the differentiation of T cells to Th1 cells, the induction of IFN gamma production by T and NK cells which block the formation of new blood vessels and the signaling pathways mediated by these cytokines play a role in inflammation, autoimmune disease and infection. IL-12p70 is the heterodimeric 70kDa cytokine made up on subunits p35 and p40. |
| Product Information | |
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| Components |
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| Applications | |
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| Application | The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-12p70 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-12p70 in human serum and plasma. |
| Key Applications |
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| Application Notes | This new streamline kit has been improved with the following features over the legacy IL-12 kit, #03-0057-00: • Improved options for sample prep and incubation • Lyophilization of the standard provides the kit at one storage condition • Removal of a plate transfer step • Increased reagent volumes to accommodate automation • Validated to run on both SMCxPRO™ and Erenna® • Matched to WHO standard |
| Biological Information | |
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| Species Reactivity |
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| Species Reactivity Note | Although IL-12p70 was detectable in rat, mouse, monkey, canine, and feline plasma, the assay has not been validated for these species. |
| Cross Reactivity | Cross-reactivity to interferons of other types was tested using SMC™ Human IL-12p70 High Sensitivity Immunoasay Kit with the following results: rhIL-12p40 was found to be < 2.0% cross-reactive rhIL-23 was found to be < 1.5% cross-reactive rhILp35 was not found to be cross-reactive |
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| Physicochemical Information | |
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| Product Usage Statements | |
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| Packaging Information | |
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| Material Size | 96 well assay |
