millipore,
密理博,17-500,Catch and Release v2.0 Reversible Immunoprecipitation System
重要规格表
| Species Reactivity | Key Applications |
|---|
| A
| IP, IPK, WB
|
| Description
|
| Catalogue Number | 17-500 |
| Brand Family |
Upstate
|
| Trade Name |
|
| Description | Catch and Release® v2.0 Reversible Immunoprecipitation System |
| Overview | Features & Benefits:
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form
Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP's, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release® is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use. |
| Product Information
|
| Components |
- Catch and Release® Wash Buffer, 10X (Cat.# 20-210)
- Catch and Release® Non-denaturing Elution Buffer, 4X (Cat.# 20-209)
- Catch and Release® Denaturing Elution Buffer, 1X (Cat.# 20-284)
- 20-285
- 20-216
- Catch and Release® Capture Tubes
|
| Quality Level | MQ100
|
| Applications
|
| Application | Catch & Release v2.0 Reversible Immunoprecipitation System to purify both native & denatured proteins using a variety of antibodies & sample types. Comes in a package of 5 immunoprecipitations. |
| Key Applications |
- Immunoprecipitation
- IP-Kinase Assay
- Western Blotting
|
| Application Notes | Catch and Release® v2.0 should not be used with proteins containing a 6-His tag. |
| Biological Information
|
| Species Reactivity |
|
| Product Usage Statements
|
| Quality Assurance | routinely evaluated by immunoprecipitation. |
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | All components to be stored at 4°C except the Catch and Release® Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment. |
| Packaging Information
|
| Material Size | 1 kit |
| Material Package | 50 immunoprecipitations |
