millipore,
密理博,03-0172-00,SMC Human Thymic Stromal Lymphopoietin (TSLP) High Sensitivity Immunoassay Kit
重要规格表
| Key Applications |
|---|
| Single Molecule Counting (SMC™)
|
| Description
|
| Catalogue Number | 03-0172-00 |
| Trade Name |
|
| Description | SMC™ Human Thymic Stromal Lymphopoietin (TSLP) High Sensitivity Immunoassay Kit |
| Overview | The SMC™ Human Thymic Stromal Lymphopoietin (TSLP) High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | TSLP is a cytokine produced by epithelial cells and keratinocytes and is critical in the maturation of T cells. TSLP has been shown to be important in diseases including asthma, airway inflammatory disease, atopic dermatitis, eczema, and other allergy related diseases. In addition to its role in allergic disorders, TSLP may also block TH1/TH17 responses which may impact multiple organ systems in disease states, including the promotion of cancer and autoimmunity. |
| Product Information
|
| Components |
- Assay Buffer
- TSLP Coated Beads
- Standard Diluent
- TSLP Detection Antibody
- TSLP Standard
- 10X Wash Buffer
- Buffer D
- Elution Buffer B
|
| Precision, % |
- Intra-assay CV% average (n=20): <5%
- Inter-assay CV% average (n=6): <12%
|
| Applications
|
| Application | The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human Thymic Stromal Lymphopoietin (TSLP) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of TSLP in human serum and plasma. |
| Key Applications |
- Single Molecule Counting (SMC™)
|
| Application Notes | • Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Removal of a plate transfer step
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO™ and Erenna® |
| Biological Information
|
| Cross Reactivity | The SMC™ Human Thymic Stromal Lymphopoietin (TSLP) High Sensitivity Immunoasay Kit was not tested for cross reactivity. |
| Accuracy |
- Minimum of 5 serum samples spiked with average recovery of 113% with a range of 105-142%
- Minimum of 5 plasma samples spiked with average recovery of 104% with a range of 81-142%
|
| Physicochemical Information
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| Sensitivity |
- Lower Limit of Quantification: 1.56 pg/mL
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| Product Usage Statements
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| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| Packaging Information
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| Material Size | 96 well assay |
