重要规格表
| Species Reactivity | Key Applications |
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| NhP, Ca, R, M | Single Molecule Counting (SMC™) |
| Description | |
|---|---|
| Catalogue Number | 03-0168-00 |
| Trade Name |
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| Description | SMC™ Human IL-5 High Sensitivity Immunoassay Kit |
| Overview | The SMC™ Human IL-5 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | Interleukin 5 (IL-5), produced by T helper cells and mast cells, is a mediator of eosinophil maturation in bone marrow and release into the bloodstream and plays a role in eosinophil-based inflammatory processes, particularly allergic inflammation, asthma, and eosinophilic esophagitis. Inhibiting the action of IL-5 can suppress tissue damage due to eosinophil accumulation during pulmonary inflammation in asthma. As well, inhibition of IL-5 has been shown to reduce blood and bronchoalveolar eosinophilia caused by allergic challenge and chronic disease. |
| Product Information | |
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| Components |
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| Applications | |
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| Application | The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-5 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-5 in human serum and plasma. |
| Key Applications |
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| Application Notes | This new streamline kit has been improved with the following features including new including new improvements over the legacy IL-5 kit, #03-0053-00: • Improved LLOQ--0.04 pg/mL in the new streamlined kit while the legacy kit is 3.91 pg/mL (LLOQs of 0.01 pg/mL have been achieved with the new kit) • Adjusted standard curve--changed to 0.01 – 50 pg/mL from 0.98 – 1000 pg/mL • Added overnight incubation as an option for this assay • Improved options for sample prep and incubation • Lyophilization of the standard provides the kit at one storage condition • Removal of a plate transfer step • Increased reagent volumes to accommodate automation • Validated to run on both SMCxPRO™ and Erenna® |
| Biological Information | |
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| Species Reactivity |
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| Species Reactivity Note | Although IL-5 was detectable in non-human primate, canine, rat, and mouse samples, the assay has not been validated for these species. |
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| Physicochemical Information | |
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| Product Usage Statements | |
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| Packaging Information | |
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| Material Size | 96 well assay |