millipore,
密理博,03-0163-00,SMC Human TNFα High Sensitivity Immunoassay Kit
重要规格表
| Species Reactivity | Key Applications |
|---|
| NhP, R, Fe, Ca, Eq
| Single Molecule Counting (SMC™)
|
| Description
|
| Catalogue Number | 03-0163-00 |
| Trade Name |
|
| Description | SMC™ Human TNFα High Sensitivity Immunoassay Kit |
| Overview | The SMC™ Human TNFα High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | TNF alpha (TNFα) is a cytokine produced by activated macrophages as well as other cells such as lymphocytes, neutrophils and mast cells. It is a key cytokine in the acute phase reaction and its primary role is regulation of immune cells. It can induce fever, inflammation and apoptotic cell death while dysregulation of TNFα has been shown to play a role in diseases such as Alzheimer’s disease, cancer, psoriasis and inflammatory bowel disease. |
| Product Information
|
| Components |
- Assay Buffer
- Human TNFα Beads
- Standard Diluent
- TNFα Detection Antibody
- TNFα Standard
- 10X Wash Buffer
- Buffer D
- Elution Buffer B
|
| Precision, % |
- Intra-assay CV% average (n=20): <15%
- Inter-assay CV% average (n=5): <20%
|
| Applications
|
| Application | The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human TNFα High Sensitivity Immunoassay Kit unparalleled performance in the quantification of TNFα in human serum and plasma. |
| Key Applications |
- Single Molecule Counting (SMC™)
|
| Application Notes | This new streamline kit has been improved with the following features while the kit performance is consistent with the legacy TNFα kit, #03-0088-00:
• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Removal of a plate transfer step
• Overnight incubation can be used
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO™ and Erenna® |
| Biological Information
|
| Species Reactivity |
- Non-human Primate
- Rat
- Feline
- Canine
- Horse
|
| Species Reactivity Note | Although TNFα was detectable in non-human primate, rat, feline, canine, and equine samples, the assay has not been validated for these species. |
| Accuracy |
- Minimum of 3 serum samples spiked with average recovery of 92% with a range of 85-97%
- Minimum of 3 plasma samples spiked with average recovery of 88% with a range of 82-91%
|
| Physicochemical Information
|
| Sensitivity |
- Lower Limit of Quantification: 0.2 pg/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Packaging Information
|
| Material Size | 96 well assay |
