millipore,
密理博,03-0160-00,SMC Human IL-1β High Sensitivity Immunoassay Kit
重要规格表
| Species Reactivity | Key Applications |
|---|
| NhP, R, Ca, Fe, Eq
| Single Molecule Counting (SMC™)
|
| Description
|
| Catalogue Number | 03-0160-00 |
| Trade Name |
|
| Description | SMC™ Human IL-1β High Sensitivity Immunoassay Kit |
| Overview | The SMC™ Human IL-1β High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | IL-1β is a member of the Interleukin 1 family of cytokines and is produced by activated macrophages. It is a critical mediator of the inflammatory response and is important in cell activities such as differentiation, proliferation and apoptosis. It is important in host defense response to infection and injury. Increases in IL-1 β are associated with some autoinflammatory syndromes, ischemic diseases, tumors, inflammation and as well as Alzheimer’s disease. |
| Product Information
|
| Components |
- Assay Buffer
- IL-1β Coated Beads
- Standard Diluent
- IL-1β Detection Antibody
- IL-1β Standard
- 10X Wash Buffer
- Buffer D
- Elution Buffer B
|
| Precision, % |
- Intra-assay CV% average (n=20): <15%
- Inter-assay CV% average (n=5): <20%
|
| Applications
|
| Application | The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-1β High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-1β in human serum and plasma. |
| Key Applications |
- Single Molecule Counting (SMC™)
|
| Application Notes | This new streamline kit has been improved with the following features while the kit performance is consistent with the legacy IL-1β kit, #03-0028-00:
• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Removal of a plate transfer step
• Overnight incubation can be used
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO™ and Erenna® |
| Biological Information
|
| Species Reactivity |
- Non-human Primate
- Rat
- Canine
- Feline
- Horse
|
| Species Reactivity Note | Although IL-1β was detectable in non-human primate, rat, canine, feline, and equine samples, the assay has not been validated for these species. |
| Accuracy |
- Minimum of 3 serum samples spiked with average recovery of 85% with a range of 78-96%.
- Minimum of 3 plasma samples spiked with average recovery of 83% with a range of 80-89%.
|
| Physicochemical Information
|
| Sensitivity |
- Lower Limit of Quantification: 0.2 pg/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Packaging Information
|
| Material Size | 96 well assay |
