millipore,
密理博,HCCBP1MAG-58K,MILLIPLEX MAP Human Circulating Cancer Biomarker Magnetic Bead Panel - Cancer Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Multiplexing Ordering Requirements | Detection Methods |
|---|
| AFP, CEA, CYFRA21-1, FGF-2, HE4, PSA Total, PSA free, HGF, IL-6, IL-8, Leptin, MIF, OPN - Osteopontin, Prolactin, SCF, sFas, sFasL, TGF-α, TNF-α, TRAIL, VEGF, CA125, CA15-3, CA19-9, β-HCG
| H
| Mplex
| Please note: Total PSA and Free PSA cannot be plexed together.
| Luminex xMAP
|
| Description
|
| Catalogue Number | HCCBP1MAG-58K |
| Trade Name |
|
| Description | MILLIPLEX MAP Human Circulating Cancer Biomarker Magnetic Bead Panel - Cancer Multiplex Assay |
| Overview | millipore's MILLIPLEX MAP Circulating Cancer Biomarker magnetic bead panel consists of 25 circulating cancer biomarkers that can be used to study a wide range of tumor types. Some biomarkers are tumor type-specific, such as prostate-specific antigen (PSA), while others, such as IL-6, have been detected in many cancers.
References:
J Alzhimers Dis. 2016 Mar 8;52(2):539-59. doi: 10.3233/JAD-150914.
The Vanderbilt Memory & Aging Project: Study Design and Baseline Cohort Overview.
Jefferson AL, Gifford KA, Acosta LM, Bell SP, Donahue MJ, Davis LT, Gottlieb J, Gupta DK, Hohman TJ, Lane EM, Libon DJ, Mendes LA, Niswender K, Pechman KR, Rane S, Ruberg FL, Su YR, Zetterberg H, Liu D. |
| Background Information | Cancer research has always focused on intracellular biomarkers, such as HER2 and B-Raf, and in recent years, more and more attention has shifted to include soluble cancer biomarkers. While the use of intracellular markers in research helps clarify the process of oncogenesis, circulating cancer biomarkers give insight into how the body responds to the presence of a tumor, the dysregulation of homeostasis and the relationship between a tumor and its environment. Study of isolated biomarkers, whether intracellular or circulating, is often inadequate to fully characterize the complex relationship between tumor and non-tumor. Consequently, multianalyte panels have become essential tools that enable researchers to measure cancer biomarkers simultaneously, teasing out these relationships and applying them to understand tumor biology. |
| Product Information
|
| Components |
- Human Circulating Cancer Biomarker Panel 1 Standard (HCC-8058)
- Human Circulating Cancer Biomarker Panel 1 Quality Controls 1 and 2 (HCC-6058)
- Serum Matrix (HCP-SM)
Note: Contains 0.08% Sodium Azide - Set of one 96-Well Plate with 2 Sealers (MAG-PLATE)
- Assay Buffer (L-AB)
Note: Contains 0.08% Sodium Azide - 10X Wash Buffer (L-WB)
Note: Contains 0.05% Proclin - Human Circulating Cancer Biomarker Panel 1 Detection Antibodies (HCC-1058 or HCC-1058-2)
- Streptavidin-Phycoerythrin (L-SAPE 4)
- Bead Diluent (LBD-4)
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- Intra Assay: 4.9 - 15.0
- Inter Assay: 4.1 - 16.2
|
| Multiplex Ordering Requirements | Please note: Total PSA and Free PSA cannot be plexed together. |
| Panel Type | MAGNETIC Circulating Cancer |
| Quality Level | MQ200
|
| Applications
|
| Application | Circulating Cancer Biomarker Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple oncology biomarkers in various tumor types in human serum, plasma and cell culture samples. |
| Key Applications |
|
| Application Notes | • This is an overnight incubation assay requiring 25 µL of 1:6 dilution plasma or serum, or 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of all or any combination of the cancer biomarkers in serum, plasma or tissue/cell lysate and culture supernatant with the following exceptions:
o Total PSA and Free PSA cannot be plexed together |
| Biological Information
|
| Species Reactivity |
|
| Analytes Available |
- AFP
- CEA
- CYFRA21-1
- FGF-2
- HE4
- PSA Total
- PSA free
- HGF
- IL-6
- IL-8
- Leptin
- MIF
- OPN - Osteopontin
- Prolactin
- SCF
- sFas
- sFasL
- TGF-α
- TNF-α
- TRAIL
- VEGF
- CA125
- CA15-3
- CA19-9
- β-HCG
|
| Assay Panel | Everything you need in a single kit. |
| Cross Reactivity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Accuracy |
|
| Physicochemical Information
|
| Sensitivity |
- Refer to kit protocol for sensitivities of individual biomarkers.
|
| Standard Curve Range |
- 0.6 - 625 U/mL: CA125, CA15-3, CA19-9
- 0.09 - 67 mU/mL: β-HCG
- 0.0006 - 500 ng/mL: All other biomarkers
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| Storage and Shipping Information
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
