millipore,
密理博,HAGE1MAG-20K,MILLIPLEX MAP Human Aging Magnetic Bead Panel 1 - Metabolism Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| CTACK, FGF-21, GDF-11, GDF-15, GnRH, IL-6, IL-10, IL-18, Jag1, Leptin
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | HAGE1MAG-20K |
| Trade Name |
|
| Description | MILLIPLEX® MAP Human Aging Magnetic Bead Panel 1 - Metabolism Multiplex Assay |
| Overview | The MILLIPLEX® MAP Human Aging Panel 1 is an 11-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, and in vitro samples: C-C motif chemokine 27 (CTACK/CCL27), Fibroblast growth factor 21 (FGF-21), Growth/differentiation factor 11 (GDF-11), Growth/differentiation factor 15 (GDF-15), Gonadoliberin (GnRH), Interleukin-6 (IL-6), Interleukin-10 (IL-10), Interleukin 18 (IL-18), Jagged 1 protein (Jag1), and Leptin.
Note: IL-10 is recommended for in vitro sample use only; do not test IL-10 in serum or plasma samples. |
| Background Information | Aging associated physiological changes and the pathological consequences of these changes make the elderly population vulnerable to many chronic diseases, including diabetes, cancers, cardiovascular and neurodegenerative diseases, etc. The complexity of the aging process has stimulated intensive research over the years. Aberrant glucose metabolism, elevated chronic inflammatory levels, progressive cellular senescence and other pathophysiological conditions have proven to be associated with the aging process.
The MILLIPLEX® MAP Human Aging Panel 1 covers multifaceted biomarkers of aging-related conditions. Monitoring these novel biomarkers would help researchers to understand the basic mechanisms that drive aging and eventually lead to improved clinical interventions for extending the human health span. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- CTACK: Intra-assay <5% CV; Inter-assay <10%
- FGF-21: Intra-assay <5% CV; Inter-assay <15%
- GDF-11: Intra-assay <10% CV; Inter-assay <15%
- GDF-15: Intra-assay <10% CV; Inter-assay <10%
- GnRH: Intra-assay <10% CV; Inter-assay <15%
- IL-6: Intra-assay <5% CV; Inter-assay <10%
- IL-10: Intra-assay <5% CV; Inter-assay <15%
- IL-18: Intra-assay <5% CV; Inter-assay <15%
- Jag1: Intra-assay <10% CV; Inter-assay <15%
- Leptin: Intra-assay <5% CV; Inter-assay <10%
|
| Panel Type | MAGNETIC Metabolism |
| Applications
|
| Application | The analytes available for this multiplex kit are: CTACK/CCL27, FGF-21, GDF-11, GDF-15, Gonadoliberin (GnRH), IL-6, IL-10, IL-18, Jagged 1 protein (Jag1), & Leptin. |
| Key Applications |
|
| Application Notes | For optimal sensitivity, use an overnight (16-18 hour) incubation. A two hour incubation can also be used. Serum and plasma samples require (recommend) a 1:2 dilution with Assay Buffer. Tissue culture supernatants may be run neat or appropriately diluted in control medium. Each well requires 25 μL of sample, standard or control. |
| Biological Information
|
| Specificity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Species Reactivity |
|
| Analytes Available |
- CTACK
- FGF-21
- GDF-11
- GDF-15
- GnRH
- IL-6
- IL-10
- IL-18
- Jag1
- Leptin
|
| Assay Panel | Everything you need in a single kit. |
| Cross Reactivity | The GDF-11 antibody pair has 2.3% cross-reactivity to Myostatin/GDF-8. |
| Accuracy |
- CTACK/CCL27 93%
- FGF-21 95%
- GDF-11 88%
- GDF-15 94%
- GnRH 84%
- IL-6 93%
- IL-10 98%
- IL-18 96%
- Jag1 98%
- Leptin 96%
|
| Physicochemical Information
|
| Sensitivity |
- MinDC+2SD
- CTACK: 17.58 pg/mL
- FGF-21: 2.39 pg/mL
- GDF-11: 0.72 ng/mL
- GDF-15: 2.12 pg/mL
- GnRH: 13.43 pg/mL
- IL-6: 4.19 pg/mL
- IL-10: 35.92 pg/mL
- IL-18: 4.16 pg/mL
- Jag1: 1.75 ng/mL
- Leptin: 14.61 pg/mL
|
| Linearity of Dilution | Contact technical support for this information |
| Standard Curve Range |
- CTACK: 20 - 80,000 pg/mL
- FGF-21: 4 - 15,000 pg/mL
- GDF-11: 1 - 4,000 ng/mL
- GDF-15: 2 - 10,000 pg/mL
- GnRH: 10 - 40,000 pg/mL
- IL-6: 2 - 10,000 pg/mL
- IL-10: 39 - 160,000 pg/mL
- IL-18: 2 - 10,000 pg/mL
- Jag1: 2 - 8,000 ng/mL
- Leptin: 49 - 200,000 pg/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
