millipore,
密理博,HADK2MAG-61K,MILLIPLEX MAP Human Adipokine Magnetic Bead Panel 2 - Endocrine Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| IL-1β, IL-6, IL-8, Insulin, Leptin, MCP-1, NGF, TNF-α
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | HADK2MAG-61K |
| Trade Name |
|
| Description | MILLIPLEX MAP Human Adipokine Magnetic Bead Panel 2 - Endocrine Multiplex Assay |
| Overview | The MILLIPLEX® MAP Human Adipokine Magnetic Bead Panel 2 is a 8-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: IL-1β, IL-6, IL-8, Insulin, Leptin, MCP-1, NGF and TNF-α. This kit uses a 96-well format, contains a lyophilized standard cocktail, 2 quality controls and can measure up to 38 serum or plasma samples in duplicate.
References:
Cephalalgia. 2016 May 18. pii: 0333102416650705.
Evidence for potential involvement of pro-inflammatory adipokines in the pathogenesis of idiopathic intracranial hypertension.
Samancı B, Samancı Y, Tüzün E, Altıokka-Uzun G, Ekizoğlu E, İçöz S, Şahin E, Küçükali Cİ, Baykan B. |
| Background Information | Previously the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete a number of hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- IL-1β: Intra-assay <10% CV Inter-assay <15% CV
- IL-6: Intra-assay <10% CV Inter-assay <15% CV
- IL-8: Intra-assay <10% CV Inter-assay <15% CV
- Insulin: Intra-assay <10% CV Inter-assay <15% CV
- Leptin: Intra-assay <10% CV Inter-assay <15% CV
- MCP-1: Intra-assay <10% CV Inter-assay <15% CV
- NGF: Intra-assay <10% CV Inter-assay <15%
- TNFα: Intra-assay <10% CV Inter-assay <20% CV
|
| Panel Type | MAGNETIC Metabolism |
| Quality Level | MQ200
|
| Applications
|
| Application | The Human Adipokine Magnetic Bead Panel 2, using the Luminex xMAP technology, enables the simultaneous analysis of nine protein biomarkers in human serum and plasma samples. |
| Key Applications |
|
| Application Notes | For optimal sensitivity, use an overnight (16-18 hour) incubation. Serum and plasma samples do not require a dilution. Each well requires 25 μL of sample, standard or control. |
| Biological Information
|
| Species Reactivity |
|
| Analytes Available |
- IL-1β
- IL-6
- IL-8
- Insulin
- Leptin
- MCP-1
- NGF
- TNF-α
|
| Assay Panel | Everything you need in a single kit. |
| Cross Reactivity | Each antibody pair in a panel is highly specific and displays insignificant cross-reactivity to other analytes in the panel. |
| Accuracy |
- IL-1β 97%
- IL-6 87%
- IL-8 87%
- Insulin 94%
- Leptin 96%
- MCP-1 101%
- NGF 107%
- TNFα 91%
|
| Physicochemical Information
|
| Sensitivity |
- See kit protocol for individual sensitives.
|
| Linearity of Dilution | Contact technical support for this information |
| Standard Curve Range |
- IL-1β: 1.3 - 20,000 pg/mL
- IL-6: 0.96 - 15,000 pg/mL
- IL-8: 0.64 - 10,000 pg/mL
- Insulin: 9.6 - 150,000 pg/mL
- Leptin: 38 - 600,000 pg/mL
- MCP-1: 1.3 - 20,000 pg/mL
- NGF: 0.64 - 10,000 pg/mL
- TNF-α: 0.64 - 10,000 pg/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
| Material Package | 96-well plate |
