millipore,
密理博,HADK1MAG-61K,MILLIPLEX MAP Human Adipokine Magnetic Bead Panel 1 - Endocrine Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| Adiponectin, Adipsin, Lipocalin-2/NGAL, PAI-1 (Total), Resistin
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | HADK1MAG-61K |
| Trade Name |
|
| Description | MILLIPLEX MAP Human Adipokine Magnetic Bead Panel 1 - Endocrine Multiplex Assay |
| Overview | The MILLIPLEX® MAP Human Adipokine Magnetic Bead Panel 1 is a 5-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: Adiponectin, Adipsin, Lipocalin-2/NGAL, Total PAI-1 and Resistin. This kit uses a 96-well format, contains a lyophilized standard cocktail, 2 quality controls and can measure up to 38 serum or plasma samples in duplicate.
References:
Cephalalgia. 2016 May 18. pii: 0333102416650705.
Evidence for potential involvement of pro-inflammatory adipokines in the pathogenesis of idiopathic intracranial hypertension.
Samancı B, Samancı Y, Tüzün E, Altıokka-Uzun G, Ekizoğlu E, İçöz S, Şahin E, Küçükali Cİ, Baykan B. |
| Background Information | The role of white adipose tissue was once thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete a number of hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. The Adipokine Adiponectin is involved in the regulation of lipid and glucose metabolism, influencing the body’s response to insulin. Adiponectin's anti-inflammatory effects on the cellular lining of blood vessel walls may help explain the association of high Adiponectin levels with the reduced risk of heart attack. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- Adiponectin: Inter-assay <10% CV Intra-assay <15% CV
- Adipsin: Inter-assay <10% CV Intra-assay <10% CV
- Lipocalin-2/NGAL: Inter-assay <10% CV Intra-assay <15% CV
- PAI-1 (total): Inter-assay <10% CV Intra-assay <20% CV
- Resistin: Inter-assay <10% CV Intra-assay <20% CV
|
| Panel Type | MAGNETIC Metabolism |
| Quality Level | MQ200
|
| Applications
|
| Application | The Human Adipokine Panel 1, using the Luminex xMAP technology, enables the simultaneous analysis of five biomarkers in human serum or plasma samples. |
| Key Applications |
|
| Application Notes | For optimal sensitivity, use an overnight (16-18 hour) incubation. Serum and plasma samples require (recommend) a 1:400 dilution with Assay Buffer. Each well requires 25 μL of sample, standard or control. |
| Biological Information
|
| Specificity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Species Reactivity |
|
| Analytes Available |
- Adiponectin
- Adipsin
- Lipocalin-2/NGAL
- PAI-1 (Total)
- Resistin
|
| Assay Panel | Everything you need in a single kit. |
| Cross Reactivity | Each antibody pair in a panel is highly specific and displays insignificant cross-reactivity to other analytes in the panel. |
| Accuracy |
- Adiponectin 89%
- Adipsin 98%
- Lipocalcin 2/NGAL 91%
- PAI-1 75%
- Resistin 87%
|
| Physicochemical Information
|
| Sensitivity |
- MinDC+2SD
- Adiponectin: 21 pg/mL
- Adipsin: 10 pg/mL
- Lipocalin-2/NGAL: 3.5 pg/mL
- PAI-1: 5.8 pg/mL
- Resistin: 4.4 pg/mL
|
| Linearity of Dilution | |
| Standard Curve Range |
- Adiponectin: 26-400,000 pg/mL
- Adipsin: 13-200,000 pg/mL
- Lipocalin-2/NGAL: 3.2-50,000 pg/mL
- PAI-1 (total): 9.6-150,000 pg/mL
- Resistin: 6.4-100,000 pg/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
| Material Package | 96-well plate |
