millipore,
密理博,HADCYMAG-61K,MILLIPLEX MAP Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| Adiponectin, HGF, IL-1β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (Total), Resistin, TNF-α
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | HADCYMAG-61K |
| Trade Name |
|
| Description | MILLIPLEX MAP Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay |
| Overview | The MILLIPLEX® MAP Human Adipocyte Magnetic Bead Panel is a 11-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cell/tissue culture samples: Adiponectin, HGF, IL-β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (total), Resistin, TNFα. |
| Background Information | The role of white adipose tissue was once thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete a number of hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism. One of these Adipokines, Adiponectin, is involved in the regulation of lipid and glucose metabolism, influencing the body’s response to insulin. Also, its anti-inflammatory effects on the cellular lining of blood vessel walls may help explain the association of high Adiponectin levels with the reduced risk of heart attack. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- Adiponectin: Intra-assay <10% CV Inter-assay <15% CV
- HGF: Intra-assay <10% CV Inter-assay <20% CV
- IL-1β: Intra-assay <15% CV Inter-assay <20% CV
- IL-6: Intra-assay <10% CV Inter-assay <20% CV
- IL-8: Intra-assay <10% CV Inter-assay <15% CV
- Leptin: Intra-assay <20% CV Inter-assay <25% CV
- MCP-1: Intra-assay <10% CV Inter-assay <15% CV
- NGF: Intra-assay <10% CV Inter-assay <20%
- PAI-1 (total): Intra-assay <10% CV Inter-assay <20% CV
- Resistin: Intra-assay <10% CV Inter-assay <15% CV
- TNFα: Intra-assay <10% CV Inter-assay <20% CV
|
| Panel Type | MAGNETIC Metabolism |
| Quality Level | MQ200
|
| Applications
|
| Application | The Human Adipocyte Magnetic Bead Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 11 Adipocyte protein biomarkers in cell culture samples. |
| Key Applications |
|
| Application Notes | For optimal sensitivity, use an overnight (16-18 hour) incubation. Tissue culture supernatants may be run neat or appropriately diluted in control medium. Each well requires 25 μL of sample, standard or control. |
| Biological Information
|
| Specificity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Species Reactivity |
|
| Analytes Available |
- Adiponectin
- HGF
- IL-1β
- IL-6
- IL-8
- Leptin
- MCP-1
- NGF
- PAI-1 (Total)
- Resistin
- TNF-α
|
| Assay Panel | Everything you need in a single kit. |
| Cross Reactivity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel. |
| Accuracy |
- Adiponectin 97%
- HGF 99%
- IL-1β 99%
- IL-6 100%
- IL-8 99%
- Leptin 90%
- MCP-1 103%
- NGF 101%
- PAI-1 (total) 83%
- Resistin 87%
- TNFα 102%
|
| Physicochemical Information
|
| Sensitivity |
- MinDC+2SD
- Adiponectin 15 pg/mL
- HGF 3.0 pg/mL
- IL-1β 2.0 pg/mL
- IL-6 0.6 pg/mL
- IL-8 0.2 pg/mL
- Leptin 10 pg/mL
- MCP-1 2.1 pg/mL
- NGF 0.2 pg/mL
- PAI-1 (total) 4.6 pg/mL
- Resistin 4.4 pg/mL
- TNFα 0.2 pg/mL
|
| Linearity of Dilution | Contact technical support for this information |
| Standard Curve Range |
- Adipnectin: 26 - 400,000 pg/mL
- HGF: 6.4 – 100,000 pg/mL
- IL-1β: 0.51 - 8,000 pg/mL
- IL-6: 0.96 - 150,000 pg/mL
- IL-8: 0.29 - 4,000 pg/mL
- Leptin: 9.6 - 150,000 pg/mL
- MCP-1: 0.96 - 150,000 pg/mL
- NGF : 0.32 - 5,000 pg/mL
- PAI-1: 9.6 - 150,000 pg/mL
- Resistin: 6.4 - 100,000 pg/mL
- TNFα: 0.38 - 6,000 pg/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
| Material Package | 96 well plate |
