| Appearance (clearness) | clear |
| Appearance (color) | yellowish-brown |
| pH-value (25 °C) | 7.2 - 7.6 |
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of E.coli and S.aureus
Additionaly monoculture of S.aureus
|
| Inoculum on reference medium (Escherichia coli ATCC 35150 (O157)) | ≤ 100 |
| Inoculum on reference medium (Escherichia coli ATCC 700728(WDCM 00014)) | ≤ 100 |
| Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)) | ≤ 100 |
| Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)) | ≥ 10000 |
| Growth (Escherichia coli ATCC 35150 (O157)) | > 10 yellowish-brown colonies on CT-SMAC agar |
| Growth (Escherichia coli ATCC 700728 (WDCM 00014)) | > 10 yellowish-brown colonies on CT-SMAC agar |
| Growth Escherichia coli
ATCC 25922 (WDCM 00013) | > 10 pink colonies on CT-SMAC agar |
| Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)) | total inhibition on TSA |
Incubation:
mTSB broth: 18 - 24 hours at 41,5 °C, aerobic
Confirmation on CT-SMAC Agar resp. TSA: 24 ± 3 hours at 37 ± 1 °C aerobic
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