millipore,
密理博,03-0058-00,SMC Human Interleukin 15 (IL-15)IMMUNOASSAY KIT
重要规格表
| Analytes Available | Species Reactivity | Key Applications |
|---|
| IL-15
| H
| Single Molecule Counting (SMC™)
|
| Description
|
| Catalogue Number | 03-0058-00 |
| Trade Name |
|
| Description | SMC™ Human Interleukin 15 (IL-15)
IMMUNOASSAY KIT |
| Overview | The SMC™ Human IL-15 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | Interleukin-15 (IL-15) is a cytokine that bears structural and biological similarities to IL-2, as both cytokines are triggered by the presence of exogenous bacteria and viruses. Unlike IL-2, which is expressed mainly by activated monocytes, IL-15 is constitutively expressed by a variety of tissues and cell types, including macrophages, dendritic cells, osteoclasts and fibroblasts. IL-15 is known to affect the proliferation of T-, B- and natural killer cells, and initiates the up-regulation of the cytokines GM-CSF and TNF-α. The pro-inflammatory properties of IL-15 elicit autoimmune activity and elevated levels of IL-15 have been associated with psoriasis, multiple sclerosis, rheumatoid arthritis and systemic lupus. |
| Product Information
|
| Components |
- IL-15 Coated Beads
- Standard Diluent
- IL-15 Detection Antibody
- Assay Buffer
- Human IL-15 Standard
- SMC™ Human IL-15 Immunoassay Kit Instructions
- 10X Wash Buffer
- Elution Buffer B
- Buffer D
|
| Precision, % |
- Low-end CV% Range: 7 - 16%
- Low-end CV% Average: 10%
|
| Quality Level | MQ200
|
| Applications
|
| Application | The sensitivity of Single Molecule Counting (SMC™) gives the SMC™ IL-15 Immunoassay Kit unparalleled performance in the quantification of IL-15 in human EDTA plasma and serum. |
| Key Applications |
- Single Molecule Counting (SMC™)
|
| Biological Information
|
| Species Reactivity |
|
| Analytes Available |
|
| Accuracy |
- Minimum of 10 samples spiked with acceptable recovery between 80-120%.
|
| Physicochemical Information
|
| Linearity of Dilution | Minimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%. |
| Product Usage Statements
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| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Packaging Information
|
| Material Size | 96 well assay |
