millipore,
密理博,03-0072-00,SMC Interleukin 1 alpha (IL-1α)IMMUNOASSAY KIT
重要规格表
| Species Reactivity | Key Applications |
|---|
| H
| Single Molecule Counting (SMC™)
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| Description
|
| Catalogue Number | 03-0072-00 |
| Trade Name |
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| Description | SMC™ Interleukin 1 alpha (IL-1α)
IMMUNOASSAY KIT |
| Overview | The Singulex® Erenna® SMC™ IL-1α Immunoassay Kit contains all reagents required to perform assays on the Erenna® Immunoassay System. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® Immunoassay System detects analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | Interleukin-1 alpha and beta (IL-1α and IL-1β) are pro-inflammatory cytokines involved in immune defense against infection, and are part of the IL-1 superfamily of cytokines. Both IL-1α and IL-1β are produced by macrophages, monocytes and dendritic cells. IL-1 is involved in various immune responses with a primary role in inflammation, making it a target for Rheumatoid Arthritis (RA). IL-1α and IL-1β are produced as precursor peptides, which are proteolytically processed and released in response to cell injury, and thus induce apoptosis. IL-1β production in peripheral tissue has also been associated with hyperalgesia (increased sensitivity to pain) associated with fever. |
| Product Information
|
| Components |
- IL-1α Coated Beads
- Standard Diluent
- IL-1α Detection Antibody
- Assay Buffer
- IL-1α Standard
- Erenna IL-1α Immunoassay Kit Instructions
- 10X Wash Buffer
- Elution Buffer B
- Buffer D
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| Precision, % |
- Low-end CV% Range: 2 - 19%
- Low-end CV% Average: 7%
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| Quality Level | MQ200
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| Applications
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| Application | The sensitivity of the Single Molecule Counting (SMC™) gives the SMC™ Human IL-1α Immunoassay Kit unparalleled performance in the quantification of IL-1α in human EDTA plasma. |
| Key Applications |
- Single Molecule Counting (SMC™)
|
| Biological Information
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| Species Reactivity |
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| Accuracy |
- Minimum of 10 samples spiked with acceptable recovery between 80-120%.
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| Physicochemical Information
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| Linearity of Dilution | Minimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%. |
| Product Usage Statements
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| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| Packaging Information
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| Material Size | 96 well assay |
