millipore,
密理博,03-0068-00,SMC Human Vascular Endothelial Growth Factor (VEGF) IMMUNOASSAY KIT
重要规格表
| Species Reactivity | Key Applications |
|---|
| H
| Single Molecule Counting (SMC™)
|
| Description
|
| Catalogue Number | 03-0068-00 |
| Trade Name |
|
| Description | SMC™ Human Vascular Endothelial Growth Factor (VEGF)
IMMUNOASSAY KIT |
| Overview | The SMC™ Human VEGF Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | VEGF-A, commonly known as VEGF, is one member of a family of secreted glycoproteins that promote endothelial cell growth, survival, migration, and vascular permeability, all of which contribute to angiogenesis. The binding of VEGF to its receptor triggers the activation of a cell signaling pathway that is critical for the growth of blood vessels from pre-existing vasculature. VEGF is implicated in a variety of diseases, including several cancers, age-related macular degeneration, diabetic retinopathy and rheumatoid arthritis. With the efficacy seen with drugs that target VEGF and its pathway, VEGF will remain an attractive candidate for the development of therapies for these diseases, particularly cancer. |
| Product Information
|
| Components |
- VEGF Coated Beads
- Standard Diluent
- VEGF Detection Antibody
- Assay Buffer
- VEGF Standard
- SMC™ Human VEGF Immunoassay Kit Instructions
- 10X Wash Buffer
- Elution Buffer B
- Buffer D
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| Precision, % |
- Low-end CV% Range: 2 - 15%
- Low-end CV% Average: 7%
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| Quality Level | MQ200
|
| Applications
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| Application | The sensitivity of Single Molecule Counting (SMC™) gives the SMC™ Human VEGF Immunoassay Kit unparalleled performance in the quantification of VEGF in human EDTA plasma. |
| Key Applications |
- Single Molecule Counting (SMC™)
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| Biological Information
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| Species Reactivity |
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| Accuracy |
- Minimum of 10 samples spiked with acceptable recovery between 80-120%.
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| Physicochemical Information
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| Linearity of Dilution | Minimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%. |
| Product Usage Statements
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| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| Packaging Information
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| Material Size | 96 well assay |
