millipore,
密理博,03-0057-00,SMC Human Interleukin 12 (IL-12) IMMUNOASSAY KIT
重要规格表
| Analytes Available | Species Reactivity | Key Applications |
|---|
| IL-12
| H
| Single Molecule Counting (SMC™)
|
| Description
|
| Catalogue Number | 03-0057-00 |
| Trade Name |
|
| Description | SMC™ Human Interleukin 12 (IL-12)
IMMUNOASSAY KIT |
| Overview | The SMC™ Human IL-12 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | Interleukin-12 (IL-12) is a cytokine expressed by T-cells and natural killer cells in response to the presence of microbial agents. Increased expression of IL-12 drives a positive feedback loop, which generates more T-cells and natural killer cells, along with other immuno-regulatory cytokines such as IFN-γ, IL-10 and TNF-α. Although IL-12 is an important initiator of cell-mediated immunity, its pro-inflammatory effects can be deleterious. Over production of this cytokine has been implicated in several autoimmune disorders such as inflammatory bowel disease, psoriasis, and Crohn’s disease. The ability to escalate and strengthen the immune response makes IL-12 an important immunostimulant therapy for infectious diseases and metastatic cancers. |
| Product Information
|
| Components |
- IL-12 Coated Beads
- Standard Diluent
- IL-12 Detection Antibody
- Assay Buffer
- Human IL-12 Standard
- SMC™ Human IL-12 Immunoassay Kit Instructions
- 10X Wash Buffer
- Elution Buffer B
- Buffer D
|
| Precision, % |
- Low-end CV% Range: 2 - 8%
- Low-end CV% Average: 4%
|
| Quality Level | MQ200
|
| Applications
|
| Application | The sensitivity of Single Molecule Counting (SMC™) gives the SMC™ Human IL-12 Immunoassay Kit unparalleled performance in the quantification of IL-12 in human EDTA plasma and serum. |
| Key Applications |
- Single Molecule Counting (SMC™)
|
| Biological Information
|
| Species Reactivity |
|
| Analytes Available |
|
| Accuracy |
- Minimum of 10 samples spiked with acceptable recovery between 80-120%.
|
| Physicochemical Information
|
| Linearity of Dilution | Minimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%. |
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Packaging Information
|
| Material Size | 96 well assay |
