millipore,
密理博,03-0055-00,SMC Human Interleukin 8 (IL-8) IMMUNOASSAY KIT
重要规格表
| Analytes Available | Species Reactivity | Key Applications |
|---|
| IL-8
| H
| Single Molecule Counting (SMC™)
|
| Description
|
| Catalogue Number | 03-0055-00 |
| Trade Name |
|
| Description | SMC™ Human Interleukin 8 (IL-8)
IMMUNOASSAY KIT |
| Overview | The SMC™ Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology. |
| Background Information | Interleukin-8 (IL-8) is a CXC chemokine synthesized by many cell types, including macrophages, epithelial cells and endothelial cells in response to a pathogen or inflammatory stimulus. IL-8 functionendothelials as a chemotactic factor that attracts neutrophils, basophils, and T-cells. IL-8 is one of the major mediators of the inflammatory response and of innate immunity. Its function is to recruit neutrophils to phagocytose the foreign antigen. IL-8 is also a potent angiogenic factor that induces neutrophils to leave the bloodstream and enter into the surrounding tissue. IL-8 has been shown to have roles in the pathological angiogenesis that is associated with solid tumors, diabetic retinopathy and rheumatoid arthritis (RA). |
| Product Information
|
| Components |
- Human IL-8 Coated Beads
- Standard Diluent
- Human IL-8 Detection Antibody
- Assay Buffer
- Human IL-8 Standard
- SMC™ Human IL-8 Immunoassay Kit Instructions
- 10X Wash Buffer
- Elution Buffer B
- Buffer D
|
| Precision, % |
- Low-end CV% Range: 6 - 18%
- Low-end CV% Average: 12%
|
| Quality Level | MQ200
|
| Applications
|
| Application | The sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ IL-8 Immunoassay Kit unparalleled performance in the quantification of IL-8 in human EDTA plasma and serum. |
| Key Applications |
- Single Molecule Counting (SMC™)
|
| Biological Information
|
| Species Reactivity |
|
| Analytes Available |
|
| Accuracy |
- Minimum of 10 samples spiked with acceptable recovery between 80-120%.
|
| Physicochemical Information
|
| Linearity of Dilution | Minimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%. |
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Packaging Information
|
| Material Size | 96 well assay |
