重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|---|---|---|
| Troponin I (TnI), Troponin T (TnT), Creatine Kinase, Muscle (CKM), FABP3, Follistatin-like Protein 1 (FSTL1), Myosin Light Chain 3 (MYL3), TIMP-1 | R | Mplex | Luminex xMAP |
| Description | |
|---|---|
| Catalogue Number | RCI1MAG-87K |
| Trade Name |
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| Description | MILLIPLEX MAP Rat Cardiac Injury Panel 1 - Cardiotoxicity Multiplex Assay |
| Overview | The MILLIPLEX® MAP Rat Cardiac Injury Magnetic Bead Panel 1 (RCI1MAG-87K) contains all the components necessary to simultaneous quantify any or all of the following 7 analytes in serum and plasma samples: • Cardiac Troponin I (cTnI) • Cardiac Troponin T (cTnT) • Creatine Kinase, Muscle (CKM) • Fatty Acid Binding Protein 3 (FABP3) • Follistatin-like Protein 1 (FSTL1) • Myosin Light Chain 3 (MYL3) • Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) This kit uses a 96-well format, contains lyophilized standard cocktail and two quality controls and can measure up to 38 serum or plasma samples in duplicate. |
| Background Information | Drug-induced cardiotoxicity can be life threatening and necessitates limiting an otherwise-effective treatment. In fact, cardiovascular toxicity has been reported to account for over 40% of drug candidate withdrawal in premarketing clinical trials. Many therapeutic agents have been reported to induce or exacerbate heart failure including anti-tumor medications, immunomodulating drugs, antidepressants and calcium channel blocking agents to name a few. Conventional biomarkers of cardiotoxicity such as aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine kinase (CK) have gradually been withdrawn due to low tissue specificity. The number of cardiac-specific biomarkers adapted from clinical studies of cardiotoxicity continues to rise. The reverse translational application of these auspicious biomarkers in experimental animal models could improve the power of detection for diagnosis, helping to predict the occurrence of cardiotoxicity in humans. Emerging biomarkers of cardiac injury include multiple categories of proteins: characteristic muscle cell proteins (cTnI, cTnT, FABP3 and MYL3), cytosolic kinase (CKM), secreted heparin-binding protein (FSTL1) and metalloprotease inhibitor (TIMP1). Numerous studies have demonstrated elevated levels of these biomarkers in experimental animal blood samples. In order to meet the increasing need to provide screening assays for experimental biomarkers of drug-induced cardiac injury in pre-clinical and translational research models, EMD millipore has developed the MILLIPLEX® MAP Rat Cardiac Injury Magnetic Bead Panel 1. This confugurable 7-plex panel is the most versatile system available for cardiac injury screening in pre-clinical and translational research models. |
| Product Information | |
|---|---|
| Components |
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| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
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| Panel Type | MAGNETIC Toxicity |
| Applications | |
|---|---|
| Application | The MILLIPLEX® MAP Rat Cardiac Injury Magnetic Bead Panel 1 is a configurable 7-plex kit for the quantification of cTnI, cTnT, CKM (MM-CK), FABP3, FSTL1, MYL3, and TIMP-1 in serum or plasma samples on the Luminex xMAP® instrument platform. |
| Key Applications |
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| Application Notes | • This assay requires 25 µL of 1:5 diluted serum or plasma sample per well in kit Assay Buffer (please dilute per protocol instructions). • This assay protocol includes instruction for 2 Hour or Overnight incubation. |
| Biological Information | |
|---|---|
| Specificity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. |
| Species Reactivity |
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| Analytes Available |
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| Assay Panel | Everything you need in a single kit. |
| Cross Reactivity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. |
| Accuracy |
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| Physicochemical Information | |
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| Sensitivity |
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| Linearity of Dilution | Contact technical support for this information. |
| Standard Curve Range |
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| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information | |
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| Material Size | 96-well plate |
