millipore,
密理博,RADPKMAG-80K,MILLIPLEX MAP Rat Adipokine Panel - Metabolism Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| IL-1β, IL-6, Insulin, Leptin, MCP-1, PAI-1 (Total), TNFα
| R
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | RADPKMAG-80K |
| Trade Name |
|
| Description | MILLIPLEX® MAP Rat Adipokine Panel - Metabolism Assay |
| Overview | The Milliplex® MAP Rat Adipokine Magnetic Bead Panel contains all the components necessary to measure 7 biomarkers in any combination: IL-1β, IL-6, Insulin, Leptin, MCP-1, PAI-1 (Total), and TNFα. The kit uses a 96-well format, contains a lyophilized standard cocktail, 2 quality controls and can measure up to 38 serum or plasma samples in duplicate. |
| Background Information | In the past, the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete a number of hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism.
The MILLIPLEX® MAP Rat Adipokine Magnetic Bead Panel contains carefully selected biomarkers, enabling researchers to focus on the therapeutic potential of adipokines, adipocytes and cytokine biomarkers simultaneously. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative detection of multiple analytes simultaneously, which can dramatically improve productivity. |
| Product Information
|
| Components |
- Rat Adipokine Standard, 1 vial, lyophilized
- Rat Adipokine Quality Control 1, 1 vial, lyophilized
- Rat Adipokine Quality Control 2, 1 vial, lyophilized
- Assay Buffer, 1 bottle, 30 mL
- Bead Diluent, 1 vial, 3.5 mL
- Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
- Rat Adipokine Detection Antibodies, 1 bottle, 5.5 mL
- Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
- Mixing Bottle, 1 bottle
- Sample Plate, 96 well format, 1 plate
- 2 Foil Plate Sealers
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- Inter-assay: < 15
- Intra-Assay: < 10
|
| Panel Type | MAGNETIC Metabolism |
| Quality Level | MQ200
|
| Applications
|
| Application | The MILLIPLEX® MAP Rat Adipokine Magnetic Bead Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: IL-1β, IL-6, Insulin, Leptin, MCP-1, PAI-1 Total, and TNFα. |
| Key Applications |
|
| Application Notes | This assay is an overnight assay. Each well requires 25 μL of sample, standard or control. A maximum of 25 μL per well of neat serum or plasma can be used. |
| Biological Information
|
| Specificity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel. |
| Species Reactivity |
|
| Analytes Available |
- IL-1β
- IL-6
- Insulin
- Leptin
- MCP-1
- PAI-1 (Total)
- TNFα
|
| Assay Panel | Everything you need in a single kit. |
| Cross Reactivity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel. |
| Accuracy |
|
| Physicochemical Information
|
| Sensitivity |
- 0.3 - 17 pg/mL (MinDC).
- Please see kit protocol for individual assay sensitivities.
|
| Linearity of Dilution | Contact technical support for this information. |
| Standard Curve Range |
- IL-1β, TNFα: 0.7 – 3,000 pg/mL
- MCP-1: 9.8 – 40,000 pg/mL
- PAI-1: 12 – 50,000 pg/mL
- IL-6, Leptin: 15 – 60,000 pg/mL
- Insulin: 24 – 100,000 pg/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
