millipore,
密理博,HPTP1MAG-66K,MILLIPLEX MAP Human Pituitary Magnetic Bead Panel 1 - Endocrine Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| ACTH, AGRP, CNTF, FSH, GH, LH, TSH
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | HPTP1MAG-66K |
| Trade Name |
|
| Description | MILLIPLEX MAP Human Pituitary Magnetic Bead Panel 1 - Endocrine Multiplex Assay |
| Overview | The MILLIPLEX® MAP Human Pituitary Magnetic Bead Panel 1 is a 7-plex kit that can be used for the simultaneous measurement of any or all of the following analytes in human serum, plasma or cell/tissue culture samples: ACTH, AGRP, CNTF, FSH, GH, LH, and TSH. |
| Background Information | Pituitary hormones and other brain-derived proteins, such as hypothalamus neuropeptides, play very important roles in the regulation of various functions including metabolism, growth, and reproduction. Accurate measurement of these proteins to understand their new biological functions and molecular mechanisms of the functions are crucial. Traditional laboratory methods, such as RIA and ELISA are not able to measure multiple proteins with a small sample volume. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- ACTH: Intra-assay <10% CV Inter-assay <15%
- AgRP: Intra-assay <10% CV Inter-assay <15%
- CNTF: Intra-assay <10% CV Inter-assay <15%
- FSH: Intra-assay <10% CV Inter-assay <15%
- GH: Intra-assay <10% CV Inter-assay <15%
- LH: Intra-assay <10% CV Inter-assay <15%
- TSH: Intra-assay <10% CV Inter-assay <15%
|
| Panel Type | MAGNETIC Endocrine |
| Quality Level | MQ200
|
| Applications
|
| Application | The Human Pituitary Panel 1, using the Luminex xMAP technology, enables the simultaneous analysis of 7 protein biomarkers in human serum, plasma and cell culture samples. |
| Key Applications |
|
| Application Notes | For optimal sensitivity, use an overnight (16-18 hour) incubation. Serum or plasma samples may be run neat or diluted with Assay Buffer. Tissue/cell culture supernatants may be run neat or appropriately diluted in control medium. Each well requires 25 μL of sample, standard or control. |
| Biological Information
|
| Specificity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Species Reactivity |
|
| Analytes Available |
|
| Assay Panel | Everything you need in a single kit. |
| Cross Reactivity | There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel. |
| Accuracy |
- ACTH 100%
- AGRP 77%
- CNTF 84%
- FSH 97%
- GH 94%
- LH 87%
- TSH 105%
|
| Physicochemical Information
|
| Sensitivity |
- minDC+2SD
- ACTH: 2.4 pg/mL
- AGRP: 1.12 pg/mL
- CNTF: 84.42 pg/mL
- FSH: 0.02 mlU/mL
- GH: 6.88 pg/mL
- LH: 0.02 mlU/mL
- TSH: 0.02 μlU/mL
|
| Linearity of Dilution | Contact technical support for this information |
| Standard Curve Range |
- ACTH: 3.0 - 12,500 pg/mL
- AGRP: 2.4 - 10,000 pg/mL
- CNTF: 122 - 500,000 pg/mL
- FSH: 0.024 - 100 mlU/mL
- GH: 2.4 - 10,000 pg/mL
- LH: 0.049 - 200 mlU/mL
- TSH: 0.039 - 160 μlU/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
| Material Package | 96 well plate |
