millipore,密理博,HPRTKMAG-01K,MILLIPLEX MAP RTK Phosphoprotein Magnetic Bead Panel- Cell Signaling Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|---|---|---|
| c-Kit, c-Met/HGFR (panTyr), EGFR (pan Tyr), ErbB2/HER2, ErbB3/HER3 (panTyr), ErbB4/HER4 (panTyr), FLT3 (pan Tyr), IGF-1R (pan Tyr), IR (panTyr), M-CSFR (pan Tyr), PDGFR-α (pan Tyr), PDGFRß (pan Tyr), TIE1 (pan Tyr), Tie2 (pan Tyr), VEGFR1 (pan Tyr), VEGFR2 (pan Tyr), VEGFR3 (pan Tyr), FGFR1 | H | Mplex | Luminex xMAP |
| Description | |
|---|---|
| Catalogue Number | HPRTKMAG-01K |
| Trade Name |
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| Description | MILLIPLEX MAP RTK Phosphoprotein Magnetic Bead Panel- Cell Signaling Multiplex Assay |
| Overview | The MILLIPLEX® MAP RTK Phosphoprotein Magnetic Bead Customizable Panel is used to detect changes in phosphorylated (panTyr) c-Kit, c-Met/HGFR, EGFR, ErbB2/HER2, ErbB3/HER3 , ErbB4/HER4 , FGFR1 , FLT3 , IGF-1R, IR, M-CSFR, PDGFRα, PDGFRβ, TIE1, TIE2, VEGFR1, VEGFR2, and VEGFR3 in cell lysates using the Luminex® system. MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of pathways. Once the panel of interest has been identified, you can rely on the quality we build into each kit to produce results you can trust. Performance criteria evaluated during the validation process include: cross-reactivity, assay CVs, kit stability, and sample behavior. In addition each kit meets stringent Quality Control criteria to ensure lot-to-lot reproducibility. The MILLIPLEX® MAP RTK Phosphoprotein Magnetic Bead Panel includes: • Positive and negative control cell lysates provided to qualify assay performance • Configurable magnetic beads to capture analytes of interest • Optimized detection antibody cocktail designed to yield consistent analyte profiles within a panel |
| Background Information | RTKs (Receptor Tyrosine Kinases) are transmembrane proteins that act as receptors for growth factors, neurotrophic factors and other extracellular signaling molecules. Upon binding extracellular ligands, RTKs can undergo receptor dimerization and tyrosine phosphorylation on specific residues in the cytoplasmic tails which then lead to RTK-mediated signal transduction. Of the greater than 500 known protein kinases in human genome, approximately 60 are members of the RTK family. They are central components of cell signaling networks and play crucial roles in normal physiological processes and disease processes ranging from diabetes to cancer. |
| Product Information | |
|---|---|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Panel Type | Cell Signaling |
| Quality Level | MQ200 |
| Applications | |
|---|---|
| Application | Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting. |
| Key Applications |
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| Application Notes | • An overnight (4°C) incubation is recommended for best results. • This assay requires 25 µL diluted cell lysate per well. • This kit must be run using Assay Buffer 1 (provided). • 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL). • This 18-plex panel , unlike most signaling kits that are fixed/premixed, is customizable so that it is possible to choose only those analytes that are wanted or needed. |
| Biological Information | |
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| Specificity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Species Reactivity |
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| Analytes Available |
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| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information | |
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| Material Size | 96-well plate |
| Material Package | 96-well plate |
