millipore,
密理博,HNS2MAG-95K,MILLIPLEX MAP Human Neuroscience Magnetic Bead Panel 2 - Neuroscience Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| Angiogenin, ApoE4, FABP3, Ferritin, Neurogranin, PRNP, TREM2
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | HNS2MAG-95K |
| Trade Name |
|
| Description | MILLIPLEX MAP Human Neuroscience Magnetic Bead Panel 2 - Neuroscience Multiplex Assay |
| Overview | EMD millipore’s MILLIPLEX® MAP Human Neuroscience Magnetic Bead Panel 2 is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cerebrospinal fluid (CSF), serum/plasma and cell/tissue culture supernatants for homogenates: Angiogenin, ApoE4, FABP3, Ferritin, Neurogranin, PRNP, TREM2 |
| Background Information | Monitoring protein biomarkers in cerebrospinal fluid (CSF) of patients with neurological disorders has been highly beneficial to understanding disease progression. While several CSF biomarkers can reproducibly distinguish normal and diseased samples, CSF is a difficult biological fluid to obtain in research studies. The need for blood-based biomarkers of AD has driven a continuous search for novel candidates. This configurable multiplex immunoassay quantitatively measures seven proteins present in both human CSF and blood plasma/serum that are involved in neurological disease. Cell/tissue culture samples may also be used in the panel. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Design your multiplex kit by choosing available analytes within this panel. |
| Precision, % |
- Angiogenin: Intra-assay:< 5% CV, Inter-assay: < 10% CV
- ApoE4: Intra-assay: < 5% CV, Inter-assay: <15% CV
- FABP3: Intra-assay: < 5% CV, Inter-assay: < 10% CV
- Ferritin: Intra-assay: < 10% CV, Inter-assay: < 15% CV
- Neurogranin: Intra-assay: < 10% CV, Inter-assay: < 15% CV
- PRNP: Intra-assay: < 5% CV, Inter-assay: < 10% CV
- TREM2: Intra-assay: <5% CV, Inter-assay: < 10% CV
|
| Panel Type | MAGNETIC Neuroscience |
| Applications
|
| Application | The analytes available for this multiplex kit are: Angiogenin, ApoE4, FABP3, Ferritin, Neurogranin, PRNP and TREM2. |
| Key Applications |
|
| Application Notes | • For Plasma/Serum a 1:10 dilution is recommended for the optimal detection of the analytes FABP3, Ferritin, Neurogranin, PRNP and TREM2.
• For Plasma/Serum a 1:200 dilution for ApoE4 and Angiogenin is recommended.
• Because the sample dilutions differ for Plasma/Serum samples ApoE4 and Angiogenin cannot be plexed in the same assay as FABP3, Ferritin, Neurogranin, PRNP and TREM2.
• CSF samples require a 1:10 dilution and so may all be plexed together
• Cell/Tissue culture supernatants or homogenates may be used neat or diluted in appropriate control medium.
• Bead diluent must be present in order to detect Angiogenin. (Biotinylated-Angiogenin detection antibody is included in the bead diluent.)
• This is an overnight (2-8C) assay requiring 25 ul of appropriately diluted sample per well. Alternative, a 2 hour room temperature (20-25C) incubation may be used, thought assay sensitivity may be lower. |
| Biological Information
|
| Species Reactivity |
|
| Analytes Available |
- Angiogenin
- ApoE4
- FABP3
- Ferritin
- Neurogranin
- PRNP
- TREM2
|
| Assay Panel | Everything you need in a single kit. |
| Cross Reactivity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. There is <5% cross-reactivity from ApoE2 and ApoE3 recombinant proteins on the ApoE4 beads. |
| Accuracy |
- Angiogenin 97%
- ApoE4 85%
- FABP3 84%
- Ferritin 98%
- Neurogranin 124%
- PRNP 96%
- TREM2 94%
|
| Physicochemical Information
|
| Sensitivity |
- MinDC + 2SD (pg/mL)
- Angiogenin: 5.8
- ApoE4: 162.8
- FABP3: 14.3
- Ferritin: 6.1
- Neurogranin: 19.9
- PRNP: 177.0
- TREM2: 11.0
|
| Standard Curve Range |
- Angiogenin: 2 - 10,000 pg/mL
- ApoE4: 244 - 1,000,000 pg/mL
- FABP3: 24 - 100,000 pg/mL
- Ferritin: 6 - 25,000 pg/mL
- Neurogranin: 5 - 20,000 pg/mL
- PRNP: 122 - 500,000 pg/mL
- TREM2: 15 - 50,000 pg/mL
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
| Packaging Information
|
| Material Size | 96-well plate |
