重要规格表
| Description
|
| Overview | rLysozyme™ Solution contains a highly
purified and stabilized recombinant lysozyme
that can be used for lysis of Gram-negative
bacteria, such as E. coli. The enzyme catalyzes the hydrolysis of N-acetylmuramide linkages in bacterial cell walls. The specific activity of rLysozyme (1700 KU/mg) for E. coli lysis is 250 times greater than that of chicken egg white lysozyme. rLysozyme is optimally active at physiological pH. Very small amounts of rLysozyme (3–5 KU/g cell paste) enhance the efficiency of protein extraction with
BugBuster®, BugBuster HT, and PopCulture®
Reagents. In the absence of protein extraction
reagents, direct lysis of E. coli can be achieved by treatment of 1.0 gram cell paste with 45–60 KU rLysozyme. The product is supplied as a ready-to-use solution at a concentration range of 27–33 KU/µl in 50% glycerol containing 50 mM Tris-HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% TRITON® X-100, pH 7.5. rLysozyme Solution is stable at –20°C.
 |
| Catalogue Number | 71110 |
| Brand Family |
Novagen®
|
| Product Information
|
| Unit of Definition | One unit of rLysozyme is defined as the amount of enzyme necessary to cause a decrease of 0.025 A₄₅₀ unit per min at 25°C in a 1-ml suspension (1 mg/ml) of Tuner™(DE3) cells in 0.5 x BugBuster diluted with 50 mM Tris-HCl, pH 7.5. |
| Quality Level | MQ400
|
| Storage and Shipping Information
|
| Ship Code |
Blue Ice Only
|
| Toxicity |
Standard Handling
|
| Storage |
-20°C
|
| Avoid freeze/thaw |
Avoid freeze/thaw
|
| Do not freeze |
Ok to freeze
|
