millipore,
密理博,69036,Factor Xa, Restriction Grade, Bovine Plasma
重要规格表
| Description
|
| Overview | Restriction Grade Factor Xa is a highly purified preparation isolated from bovine plasma and activated with Russell's viper venom. This preparation is purified to near homogeneity and shows no secondary cleavage from contaminating proteases. The preparation is also functionally tested for activity with fusion proteins. Like enterokinase, Factor Xa cleaves at the C-terminal side of the recognition sequence (IleGluGlyArg↓) and can therefore be used for removing all vector-encoded sequences from appropriately designed constructs.
Specific Activity: ≥150 IU/mg
One IU is defined as the amount of enzyme that will release 1 µmole of p-nitroaniline per minute using methanesulfonyl-D-leucyl-glycyl-arginyl-paranitroanilide at 37°C, pH 8.4.
One µg of enzyme cleaves 50 µg Cleavage Control Protein to >95% completion in 16 hr at 25°C in a buffer containing 50 mM Tris-HCl pH 8.0, 100 mM NaCl, and 5 mM CaCl2. |
| Catalogue Number | 69036 |
| Brand Family |
Novagen®
|
| Product Information
|
| • | 100 µg | Restriction Grade Factor Xa
|
| • | 2 ml | Factor Xa Dilution/Storage Buffer
|
| • | 1 ml | 10X Factor Xa Cleavage Buffer
|
| • | 10 µg | Cleavage Control Protein |
| Biological Information
|
| Biological activity | One microgram of enzyme cleaves 50 µg Xa Cleavage Control Protein to >95% completion in 16 hours at 25°C in a buffer containing 50 mM Tris-HCl pH 8.0, 100 mM NaCl, and 5 mM CaCl₂. |
| Storage and Shipping Information
|
| Ship Code |
Blue Ice Only
|
| Toxicity |
Multiple Toxicity Values, refer to MSDS
|
| Storage |
-20°C
|
| Avoid freeze/thaw |
Avoid freeze/thaw
|
| Do not freeze |
Ok to freeze
|
