| Overview | Recombinant type 14 3C protease from
human rhinovirus (HRV 3C) is a recombinant
restriction-grade protease. HRV 3C is a highly
purified recombinant 6XHis-fusion protein,
that recognizes the same cleavage site as
the native enzyme: LeuGluValLeuPheGln↓GlyPro. The small, 22-kDa size of the protease,
optimal activity at 4°C, high specificity, and
His•Tag® fusion make HRV 3C protease an
ideal choice for rapid removal of fusion
tags. The pET expression vectors pET-47b(+)
through pET-50b(+) incorporate the HRV 3C
protease cleavage site in combinations with
His•Tag, S•Tag™, thioredoxin (Trx•Tag™),
glutathione-S-transferase (GST•Tag™),
and NusA (Nus•Tag™) sequences. The
combination of pET-47b(+) to pET-50b(+)
vectors for expression, HRV 3C protease for
fusion tag cleavage, and Ni-NTA His•Bind®
metal affinity chromatography for protein
purification as well as protease and fusion tag
removal allows production of recombinant
proteins free of vector-encoded sequences.
Other expression vectors, including the
pET-52b(+) vector, the InsectDirect™ pIEx™-9
vector, and the multisystem pTriEx™-6 vector,
all incorporate the HRV 3C protease cleavage
site in combination with the Strep•Tag® II and
His•Tag sequences. Supplied as: 1.8-3.0 U/µl in
50 mM Tris-HCl (pH 8.0), 150 mM NaCl,
1 mM EDTA, 0.5 mM THP, and 50% glycerol.
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