millipore,
密理博,508838,Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested - CAS 58-58-2 - Calbiochem
重要规格表
| Empirical Formula | CAS # |
|---|
| C₂₂H₂₉N₇O₅ • 2HCl
| 58-58-2
|
| Description
|
| Overview | An aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor. Resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the A site of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Useful for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Recommended range for selection of transfected mammalian cells ranges from 1 to 30 µg/ml. |
| Catalogue Number | 508838 |
| Brand Family |
Calbiochem®
|
| Synonyms | (2S)-2-Amino-N-((2S,3S,4R,5R)-5-(6-(dimethylamino)purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl)-3-(4-methoxyphenyl) |
| References
|
| References | Chow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
de la Luna, S. and Ortin, J. 1992. Methods Enzymol. 216, 376.
Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149. |
| Product Information
|
| CAS number | 58-58-2 |
| Form | Liquid |
| Formulation | Supplied at 10 mg/ml in H₂O. |
| Hill Formula | C₂₂H₂₉N₇O₅ • 2HCl |
| Chemical formula | C₂₂H₂₉N₇O₅ • 2HCl |
| Reversible | Y |
| Quality Level | MQ200
|
| Biological Information
|
| Primary Target | RNA |
| Purity | ≥98% by HPLC. |
| Physicochemical Information
|
| Contaminants | Endotoxin: ≤10 EU/mg dry weight |
| Storage and Shipping Information
|
| Ship Code |
Dry Ice Only
|
| Toxicity |
Standard Handling
|
| Storage |
-20°C
|
| Protect from Light |
Protect from light
|
| Avoid freeze/thaw |
Avoid freeze/thaw
|
| Do not freeze |
Ok to freeze
|
| Special Instructions | Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C. |
| Packaging Information
|
| Packaged under inert gas |
Packaged under inert gas
|
