millipore,
密理博,505156,SUMOylation Inhibitor III, 2-D08 - Calbiochem
重要规格表
| Empirical Formula |
|---|
| C₁₅H₁₀O₅
|
| Description
|
| Overview | A cell-permeable trihydroxyflavone compound that prevents the transfer of SUMO (Cat. Nos. 662037, 662039, and 662042) from the E2 thioester to the substrate without affecting SUMO-activating enzyme E1 (SAE-1/2; Cat. Nos. 662073 and 662074) or E2 Ubc9-SUMO thioester formation. A 6 h pretreatment with 100 µM 2-D08 is shown to effectively inhibit 10 µM CPT- (Camptothecin; Cat. No. 208925) induced Topoisomerase I SUMOylation in breast cancer ZR-75-1 and BT-474 cultures without affecting overall cellular protein ubiquitinations. Anacardic and Ginkgolic Acid (Cat. No. 172050 and 345887, respectively) in comparison, both inhibit E1-SUMO thioester formation instead. |
| Catalogue Number | 505156 |
| Brand Family |
Calbiochem®
|
| Synonyms | 2-(2,3,4-Trihydroxyphenyl)-4H-chromen-4-one, SUMO E2 Ubc9 Inhibitor, 2D08 |
| References
|
| References | Kim, Y.S., et al. 2013. Chem. Biol. 20, 604.
Cotelle, N., et al. 1996. Free Radic. Biol. Med. 20, 35. |
| Product Information
|
| Form | Orange-brown powder |
| Hill Formula | C₁₅H₁₀O₅ |
| Chemical formula | C₁₅H₁₀O₅ |
| Reversible | Y |
| Quality Level | MQ300
|
| Biological Information
|
| Primary Target | SUMO E2-thioester complex |
| Purity | ≥95% by HPLC |
| Physicochemical Information
|
| Cell permeable | Y |
| Storage and Shipping Information
|
| Ship Code |
Ambient Temperature Only
|
| Toxicity |
Standard Handling
|
| Storage |
+2°C to +8°C
|
| Protect from Light |
Protect from light
|
| Do not freeze |
Ok to freeze
|
| Special Instructions | Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. |
| Packaging Information
|
| Packaged under inert gas |
Packaged under inert gas
|
