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密理博,48-690MAG,MILLIPLEX MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex - Cell Signaling Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| CD3ε (pan Tyr), CREB (S133), ERK (Thr185/Tyr187), LAT (pan Tyr), Lck (pan Tyr), Syk (pan Tyr), ZAP-70 (pan Tyr)
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | 48-690MAG |
| Trade Name |
|
| Description | MILLIPLEX MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex - Cell Signaling Multiplex Assay |
| Overview | The MILLIPLEX® MAP 7-Plex Human T-Cell Receptor (TCR) Magnetic Bead Kit is used to detect changes in phosphorylated CREB (Ser133), Erk/MAP kinase 1/2 (Thr185/Tyr187), and phosphorylated tyrosine residues on CD3 epsilon chain, Lck, ZAP-70, LAT, and Syk in T-cell lysates using the Luminex® system. Although the protein kinase Syk is not part of the T-Cell Receptor pathway, it does play a significant role in B-Cell Receptor signaling and acts as a negative control for the analysis of T-Cell Receptor signaling. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Premixed |
| Precision, % |
- Intra-Assay: 3% - 8%
- Inter-Assay: 4% - 17%
|
| Panel Type | Cell Signaling |
| Quality Level | MQ200
|
| Applications
|
| Application | This cell signaling panel includes the following analytes in this multiplex kit: CD3ε (pan Tyr), CREB (S133), ERK (Thr185/Tyr187), LAT (pan Tyr), Lck (pan Tyr), Syk (pan Tyr), ZAP-70 (pan Tyr). |
| Key Applications |
|
| Application Notes | • An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well). |
| Biological Information
|
| Specificity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Species Reactivity |
|
| Analytes Available |
- CD3ε (pan Tyr)
- CREB (S133)
- ERK (Thr185/Tyr187)
- LAT (pan Tyr)
- Lck (pan Tyr)
- Syk (pan Tyr)
- ZAP-70 (pan Tyr)
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Storage and Shipping Information
|
| Storage Conditions | Recommended storage for kit components is 2 - 8°C |
| Packaging Information
|
| Material Size | 96-well plate |
