millipore,密理博,48-680MAG,MILLIPLEX MAP Multi-Pathway Magnetic Bead 9-Plex - Cell Signaling Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|---|---|---|
| CREB (pS133), ERK (pT185/pY187), NFκB (pS536), JNK (pT183/pY185), p38 (pT180/pY182), p70 S6K (pT412), STAT3 (pS727), STAT5A/B (pY694/699), Akt (pS473) | H | Mplex | Luminex xMAP |
| Description | |
|---|---|
| Catalogue Number | 48-680MAG |
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| Description | MILLIPLEX MAP Multi-Pathway Magnetic Bead 9-Plex - Cell Signaling Multiplex Assay |
| Overview | The MILLIPLEX® MAP 9-plex Multi-Pathway Signaling Magnetic Bead kit, phosphoprotein, is used to detect changes in phosphorylated ERK/MAP kinase 1/2 (Thr185/Tyr187), Akt (Ser473), STAT3 (Ser727), JNK (Thr183/Tyr185), p70 S6 kinase (Thr412), NFkB (Ser536), STAT5A/B (Tyr694/699), CREB (Ser133), and p38 (Thr180/Tyr182) in cell lysates using the Luminex® xMAP® technology. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay. References: Pharmacol Res. 2016 Sep 12;113(Pt A):356-363. doi: 10.1016/j.phrs.2016.08.028. [Epub ahead of print] The endocannabinoid anandamide causes endothelium-dependent vasorelaxation in human mesenteric arteries. Stanley CP, Hind WH, Tufarelli C, O'Sullivan SE. PLoS One. 2015 Sep 28;10(9):e0136546. doi: 10.1371/journal.pone.0136546. eCollection 2015. The Effects of the Endocannabinoids Anandamide and 2-Arachidonoylglycerol on Human Osteoblast Proliferation and Differentiation. Smith M, Wilson R, O'Brien S, Tufarelli C, Anderson SI, O'Sullivan SE. Cardiovasc Res. 2015 Sep 1;107(4):568-78. doi: 10.1093/cvr/cvv179. Epub 2015 Jun 19. Cannabidiol causes endothelium-dependent vasorelaxation of human mesenteric arteries via CB1 activation. Stanley CP, Hind WH, Tufarelli C, O'Sullivan SE. |
| Product Information | |
|---|---|
| Detection method | Luminex xMAP |
| Configuration | Premixed |
| Panel Type | Cell Signaling |
| Quality Level | MQ200 |
| Applications | |
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| Application | Analyze a cell signaling panel using this kit. The analytes available for the cell signaling multiplex assay kit are: CREB (pS133), ERK (pT185/pY187), NFκB (pS536), JNK (pT183/pY185), p38 (pT180/pY182), p70 S6K (pT412), STAT3 (pS727), STAT5A/B (pY694/699), Akt (pS473). |
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| Biological Information | |
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| Specificity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
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| Product Usage Statements | |
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| Storage and Shipping Information | |
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C |
| Packaging Information | |
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| Material Size | 96-well plate |
