millipore,
密理博,48-660MAG,MILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay
重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|
| ATF2 (Thr71), Erk (Thr185/Tyr187), HSP27 (Ser78), JNK (Thr183/Tyr185), c-Jun (Ser73), MEK1 (Ser222), MSK1 (Ser212), p38 (Thr180/Tyr182), p53 (Ser15), STAT1 (Tyr701)
| H
| Mplex
| Luminex xMAP
|
| Description
|
| Catalogue Number | 48-660MAG |
| Trade Name |
|
| Description | MILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay |
| Overview | Cells respond to their environment in various ways through intracellular signaling. Signaling through Receptor Tyrosine kinases (RTKs) often promotes increased metabolism and cell growth. Activation of ERK/MAP kinase is one of the key serine/threonine kinases activated via RTK signaling, promoting increased activity of p70 S6 kinase, MSK1, STATs, CREB, and many other signaling intermediates. Other signaling pathways induced via stress (ex. heat shock/arsenite treatment) or death receptors (ex. TNFα) promote p38, JNK, and p53 signaling pathways. |
| Product Information
|
| Detection method | Luminex xMAP |
| Configuration | Premixed |
| Precision, % |
- Intra-Assay: 3% - 10%
- Inter-Assay: 4% - 13%
|
| Panel Type | Cell Signaling |
| Quality Level | MQ200
|
| Applications
|
| Application | Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples. |
| Key Applications |
|
| Application Notes | • An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well). |
| Biological Information
|
| Specificity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
| Species Reactivity |
|
| Analytes Available |
- ATF2 (Thr71)
- Erk (Thr185/Tyr187)
- HSP27 (Ser78)
- JNK (Thr183/Tyr185)
- c-Jun (Ser73)
- MEK1 (Ser222)
- MSK1 (Ser212)
- p38 (Thr180/Tyr182)
- p53 (Ser15)
- STAT1 (Tyr701)
|
| Product Usage Statements
|
| Usage Statement |
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|
| Packaging Information
|
| Material Size | 96-well plate |
