| Appearance (colour) | brown |
| Appearance (clearness) | clear |
| pH-value (25 °C) | 7.2 - 7.6 |
| Growth promotion test in accordance with the current version of DIN EN ISO 11133. |
| Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)) | ≤ 100 |
| Inoculum on reference medium (Streptococcus pneumoniae ATCC 6305) | ≤ 100 |
| Inoculum on reference medium (Streptococcus pyogenes ATCC 19615) | ≤ 100 |
| Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)) | ≤ 100 |
| Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)) | ≤ 100 |
| Inoculum on reference medium (Bacteroides fragilis ATCC 25285) | ≤ 100 |
| Inoculum on reference medium (Haemophilus influenzae ATCC 33391) | ≤ 100 |
| Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)) | good to very good |
| Growth (Streptococcus pneumoniae ATCC 6305) | good to very good |
| Growth (Streptococcus pyogenes ATCC 19615) | good to very good |
| Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)) | good to very good |
| Growth (Candida albicans ATCC 10231 (WDCM 00054)) | good to very good |
| Growth (Bacteroides fragilis ATCC 25285) | good to very good |
| Growth (Haemophilus influenzae ATCC 33391) | good to very good |
Incubation: 24 ± 2 hours at 37 ± 1 °C aerobic C.albicans 44 ± 4 hours at 37 ± 1 °C aerobic B.fragilis 44 ± 4 hours at 37 ± 1 °C anaerobic H.influenza 44 ± 4 hours at 37 ± 1 °C microaerophilic and with addition of X+V-factor. |