| Appearance (clearness) | clear |
| Appearance (color) | yellowish-brown |
| pH-value (25 °C) | 6.6 - 7.0 |
| Growth promotion test in accordance with the current version of DIN EN ISO 11133. |
| Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)) | ≤ 100 |
| Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)) | ≤ 100 |
| Inoculum on reference medium (Citrobacter freundii ATCC 43864 (WDCM 00006)) | ≤ 100 |
| Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)) | > 10⁴ |
| Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)) | > 10⁴ |
| Growth (Escherichia coli ATCC 25922 (WDCM 00013)) | + |
| Growth (Escherichia coli ATCC 8739 (WDCM 00012)) | + |
| Growth (Citrobacter freundii ATCC 43864 (WDCM 00006)) | + |
| Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)) | none |
| Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)) | none |
| Gas (Escherichia coli ATCC 25922 (WDCM 00013)) | + |
| Gas (Escherichia coli ATCC 8739 (WDCM 00012)) | + |
| Gas (Citrobacter freundii ATCC 43864 (WDCM 00006)) | + |
| Gas (Enterococcus faecalis ATCC 19433 (WDCM 00009)) | - |
| Gas (Enterococcus faecalis ATCC 29212 (WDCM 00087)) | - |
Incubation:
Escherichia coli: 24 ± 2 hours at 30 ± 1 °C and parallel 24 ± 2 hours at 37 ± 1 °C
Citrobacter: 24 ± 2 hours at 30 ± 1 °C
Enterococcus faecalis: 48 ± 2 hours at 30 ± 1 °C and parallel 48 ± 2 hours at 37 ± 1 °C |
